Oba T, Andachi Y, Muto A, Osawa S
Department of Biology, School of Science, Nagoya University, Japan.
Biochimie. 1991 Jul-Aug;73(7-8):1109-12. doi: 10.1016/0300-9084(91)90153-r.
The in-frame UGA codons in the synthetic messenger RNA were translated in the cell-free system of Mycoplasma capricolum. The result, together with the occurrence of codon UGA at tryptophan sites in the genes and the presence of tRNA(UCATrp) pairing with UGA, clearly indicated that UGA is a tryptophan codon in this bacterium.
合成信使核糖核酸中的可读框UGA密码子在山羊支原体无细胞系统中被翻译。该结果,连同基因中色氨酸位点上UGA密码子的出现以及与UGA配对的tRNA(UCATrp)的存在,清楚地表明UGA在这种细菌中是一个色氨酸密码子。