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[外源化学激活剂作用下黄瓜的耐盐性及其机制]

[Salt resistance and its mechanism of cucumber under effects of exogenous chemical activators].

作者信息

Song Shiqing, Liu Wei, Guo Shirong, Shang Qingmao, Zhang Zhigang

机构信息

Department of Horticulture and Gardening, Hebei Normal UNiversity of Science and Technology, China.

出版信息

Ying Yong Sheng Tai Xue Bao. 2006 Oct;17(10):1871-6.

Abstract

With root injection and foliar spray, this paper studied the effects of different concentrations salicylic acid, brassinolide, chitosan and spermidine on the growth, morphogenesis, and physiological and biochemical characters of cucumber ( Cucumis sativus L. ) seedlings under 200 mmol x L(-1) NaCl stress. The results showed that at proper concentrations, these four exogenous chemical activators could markedly decrease the salt stress index and mortality of cucumber seedlings, and the decrement induced by 0. 01 mg x L (-1) brassinolide was the largest, being 63. 0% and 75. 0% , respectively. The activities of superoxide dismutase (SOD) , peroxidase (POD) and catalase (CAT) increased significantly, resulting in a marked decrease of malondialdehyde (MDA) content and electrolyte leakage. The dry weight water content and morphogenesis of cucumber seedlings improved, and the stem diameter, leaf number, and healthy index increased significantly. All of these suggested that exogenous chemical activators at proper concentrations could induce the salt resistance of cucumber, and mitigate the damage degree of salt stress. The salt resistance effect of test exogenous chemical activators decreased in the sequence of 0.005 -0.05 mg (L-1) brassinolide, 150 -250 mg x L (-1) spermidine, 100 -200 mg x L(-1) chitosan, and 50 -150 mg x L(-1) salicylic acid.

摘要

采用根部注射和叶面喷施的方法,研究了在200 mmol·L⁻¹ NaCl胁迫下,不同浓度的水杨酸、油菜素内酯、壳聚糖和亚精胺对黄瓜(Cucumis sativus L.)幼苗生长、形态建成以及生理生化特性的影响。结果表明,在适宜浓度下,这四种外源化学激活剂可显著降低黄瓜幼苗的盐胁迫指数和死亡率,其中0.01 mg·L⁻¹油菜素内酯诱导的降幅最大,分别为63.0%和75.0%。超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性显著增加,导致丙二醛(MDA)含量和电解质渗漏显著降低。黄瓜幼苗的干重、含水量和形态建成得到改善,茎粗、叶片数和健康指数显著增加。所有这些表明,适宜浓度的外源化学激活剂可诱导黄瓜的耐盐性,减轻盐胁迫的伤害程度。供试外源化学激活剂的耐盐效果依次为0.005 - 0.05 mg·L⁻¹油菜素内酯、150 - 250 mg·L⁻¹亚精胺、100 - 200 mg·L⁻¹壳聚糖和50 - 150 mg·L⁻¹水杨酸。

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