Hase M, Yamada A, Hamada T, Baigl D, Yoshikawa K
Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
Langmuir. 2007 Jan 16;23(2):348-52. doi: 10.1021/la0618521.
Cell-sized water droplets coated by a phospholipid layer mimicking the inner surface of living cells were manipulated by laser tweezers and used as biochemical microreactors. The cell-sized phospholipid-coated microdroplets (CPMDs) consisted of a water droplet in mineral oil with a diameter of 1-100 microm and coated by 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine. We monitored the time development of biochemical reactions in a single CPMD obtained after the controlled fusion of two CPMDs containing a substrate and an enzyme, respectively. We present results on two enzymatic reactions: calcein production in the presence of esterase and green fluorescence protein expression.
通过激光镊子操控由模仿活细胞内表面的磷脂层包裹的细胞大小的水滴，并将其用作生化微反应器。细胞大小的磷脂包被微滴（CPMDs）由直径为1-100微米的矿物油中的水滴组成，并由1,2-二油酰基-sn-甘油-3-磷酸乙醇胺包被。我们监测了分别含有底物和酶的两个CPMDs在受控融合后得到的单个CPMD中生化反应随时间的发展。我们展示了两种酶促反应的结果：酯酶存在下的钙黄绿素生成和绿色荧光蛋白表达。
