Borek Dominika, Ginell Stephan L, Cymborowski Marcin, Minor Wladek, Otwinowski Zbyszek
Department of Biochemistry, University of Texas, Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, TX 75390, USA.
J Synchrotron Radiat. 2007 Jan;14(Pt 1):24-33. doi: 10.1107/S0909049506046589. Epub 2006 Dec 15.
During diffraction experiments even cryo-cooled protein crystals can be significantly damaged due to chemical and physical changes induced by absorbed X-ray photons. The character and scale of the observed effects depend strongly on the temperature and the composition of crystals. The absorption of radiation energy results in incremental regular changes to the crystal structure, making its impact on the process of solving the structure strongly correlated with other experimental variables. An understanding of all the dependencies is still limited and does not allow for a precise prediction of the outcome of a particular diffraction experiment. Results are presented of diffraction experiments performed under different experimental conditions. The influence of temperature and crystal composition on different characteristics of radiation damage is analyzed. The observed effects are discussed in terms of their impact on data processing and phasing procedures.
在衍射实验中，即使是经低温冷却的蛋白质晶体，也可能因吸收X射线光子引发的化学和物理变化而受到显著损伤。所观察到的效应的特征和程度在很大程度上取决于晶体的温度和组成。辐射能量的吸收会导致晶体结构发生渐进的规律性变化，使其对结构解析过程的影响与其他实验变量密切相关。对所有这些相关性的理解仍然有限，无法精确预测特定衍射实验的结果。本文展示了在不同实验条件下进行的衍射实验结果。分析了温度和晶体组成对辐射损伤不同特征的影响。从其对数据处理和相位确定程序的影响方面对观察到的效应进行了讨论。
