Kudo Yuki, Nakahara Takako, Nakagama Tatsuro, Seino Nobuko, Shinoda Masaki, Uchiyama Katsumi
Faculty of Urban Environmental Sciences, Tokyo Metropolitan University, Tokyo, Japan.
Anal Sci. 2007 Jan;23(1):91-5. doi: 10.2116/analsci.23.91.
A surface-reaction system in a nanoliter water pool using an ink-jet microchip was developed. The reaction system in the nanodroplets formed on a poly(dimethylsiloxane) (PDMS) coated glass slide increased the diffusion-controlled reaction without using a nano-pump, specialized connector or highly sensitive detector. When nanoliter droplets were placed on the PDMS surface with a distance of 100 microm between them by the ink-jet microchip, the repeatabilities of the fluorescence intensity were 2.9% RSD (n = 7). The used ink-jet microchip had 4 different injection ports, and the distance between the ports was 0.995 mm. It was necessary to correct the distance in order to mix or dilute samples in a small droplet. The correction was successfully performed by moving the X-Y stage using inhouse-made software. A linear relationship was obtained between the Resorufin concentrations and the fluorescence intensity. We applied this system to an enzyme-linked immunosorbent assay (ELISA) for immunoglobulin A (IgA), and observed a difference in the fluorescence intensity derived from the amount of IgA (blank, 6.25 ng/mL, 12.5 ng/mL). These results show the usefulness of the open-type micro-analytical systems proposed by us.
开发了一种使用喷墨微芯片的纳升水池中的表面反应系统。在涂有聚二甲基硅氧烷（PDMS）的载玻片上形成的纳米液滴中的反应系统，在不使用纳米泵、专用连接器或高灵敏度检测器的情况下增加了扩散控制反应。当通过喷墨微芯片将纳升液滴以100微米的间距放置在PDMS表面上时，荧光强度的重复性为2.9%相对标准偏差（n = 7）。所使用的喷墨微芯片有4个不同的进样口，进样口之间的距离为0.995毫米。为了在小液滴中混合或稀释样品，有必要校正距离。通过使用自制软件移动X-Y平台成功进行了校正。在试卤灵浓度和荧光强度之间获得了线性关系。我们将该系统应用于免疫球蛋白A（IgA）的酶联免疫吸附测定（ELISA），并观察到源自IgA量（空白、6.25 ng/mL、12.5 ng/mL）的荧光强度差异。这些结果表明了我们提出的开放式微分析系统的实用性。
