Nishiyama Takahide, Endo Fumihiro, Eguchi Hiroko, Nakagama Tatsuro, Seino Nobuko, Shinoda Masaki, Shimosaka Takuya, Hobo Toshiyuki, Uchiyama Katsumi
Department of Applied Chemistry, Graduate Schools of Urban Environmental Sciences, Tokyo Metropolitan University, Tokyo, Japan.
Anal Sci. 2007 Apr;23(4):389-93. doi: 10.2116/analsci.23.389.
An ultra-micro sample injector for gas chromatography (GC) was developed. An ink-jet microchip, originally used for industrial recorder, was modified at the edge near to an orifice, and fixed into the GC. In order to evaluate the characteristics of this injector, a sample injector and a thermal conductive detector (TCD) were connected directly, while water was used as the test sample. The volume of the droplet, the interval time and the back-pressure to the ink-jet microchip were investigated. Within the range of 1 - 5 nL volume injected sample, the TCD response according to the amount of the sample volume (the volume of one droplet from the ink-jet microchip was about 1 nL) was obtained. A good reproducibility of the peak area was obtained to be about 1.0% of the RSD value. In order to compare the injection method of the ink-jet chip with that using a micro-syringe, the method using the ink-jet chip could introduce 1/1000 of the amount of the sample and gave reproducible results.
开发了一种用于气相色谱（GC）的超微样品注射器。对原本用于工业记录仪的喷墨微芯片在靠近孔口的边缘进行了改造，并将其固定到气相色谱仪中。为了评估该注射器的特性，将一个样品注射器和一个热导检测器（TCD）直接相连，同时使用水作为测试样品。研究了液滴体积、间隔时间以及作用于喷墨微芯片的背压。在进样体积为1 - 5 nL的范围内，获得了根据样品体积量（来自喷墨微芯片的一个液滴体积约为1 nL）的热导检测器响应。峰面积的重现性良好，相对标准偏差（RSD）值约为1.0%。为了将喷墨芯片的进样方法与使用微量注射器的方法进行比较，使用喷墨芯片的方法进样量可低至其千分之一，且结果具有重现性。
