el-Barrawy M A, Ismail K A, el-Barrawi S A, Sultan A A
Microbiology & Immunology, HIPH, University of Alexandria.
J Egypt Public Health Assoc. 1996;71(3-4):285-307.
Sixty seven cases of human oral S.C.C. with its intra oral variances were investigated microbiologically, histopathologically, histochemically and immunologically. Yeasts were isolated from 85% of examined cases. "Candifast test" showed that Candida albicans was the commonest form of the detected fungi, followed by C. parapsilosis and C. tropicalis, while the least in frequency was the Torulopsis glabrata. Yeasts were more detected in females, elders and tobacco smokers. Two cases of well differentiated S.C.C. were surprisingly detected, and for the first time in literature, associated with a specific granulomatous reaction and showed positively impregnated fungi. While negative reactions for fungi were noticed in all lymphoepitheliomas, almost all the verrucous carcinoma were positive. Viral inclusion bodies were demonstrated for the first time by MT. This method was rapid, economic and could be used as a pilot study before applying the more specific monoclonal antibody techniques or in-situ hybridization methods. Comparing the results of HSV hybridization with that of candida infection we found that both could be detected in some cases. The results indicate that viral and fungal factors may be synergetic in the development of oral carcinomas.
对67例口腔鳞状细胞癌及其口腔内变异情况进行了微生物学、组织病理学、组织化学和免疫学研究。85%的受检病例中分离出酵母。“Candifast检测”表明，白色念珠菌是检出真菌中最常见的形式，其次是近平滑念珠菌和热带念珠菌，而光滑假丝酵母的检出频率最低。女性、老年人和吸烟者中酵母检出率更高。令人惊讶的是，发现了2例高分化鳞状细胞癌，且在文献中首次发现其与一种特定的肉芽肿反应相关，并显示有阳性浸染的真菌。虽然在所有淋巴上皮瘤中均未发现真菌反应，但几乎所有疣状癌均呈阳性。MT首次证实了病毒包涵体。该方法快速、经济，可在应用更特异的单克隆抗体技术或原位杂交方法之前作为初步研究。比较单纯疱疹病毒杂交结果与念珠菌感染结果，我们发现在某些病例中两者均可检出。结果表明，病毒和真菌因素在口腔癌的发生发展中可能具有协同作用。
