Nelson Peter T, Zhang Paul J, Spagnoli Giulio C, Tomaszewski John E, Pasha Theresa L, Frosina Denise, Caballero Otavia L, Simpson Andrew J G, Old Lloyd J, Jungbluth Achim A
Sanders-Brown Center on Aging and Dept of Pathology, University of Kentucky, Lexington, KY, USA.
Cancer Immun. 2007 Jan 12;7:1.
Cancer/testis (CT) antigens are named after their expression pattern as they are typically present in various types of tumors and in the germ cells of normal adult testis. Adult ovarian tissue is usually reported to be CT antigen negative. Based on the differences in female versus male gonadal development, the ovarian counterpart of the most predominant CT antigen positive testicular germ cells are not prevalent in the adult ovary. Hence, we analyzed the protein expression of several CT antigens in fetal ovary by immunohistochemistry with various monoclonal antibodies (mAbs) previously generated by our group. The mAbs used were: MA454 (MAGE-A1), M3H67 (MAGE-A3), 57B (MAGE-A4), CT7-33 (CT7/MAGE-C1), and ES121 (NY-ESO-1). All mAbs showed some immunopositivity in fetal ovarian germ cells. The most intense staining was seen with mAbs M3H67, 57B, and CT7-33 during weeks 16-23 of gestation. The most prevalent cells stained were oogonia, with only focal staining of oocytes of the primordial follicle. We conclude that CT antigens are regularly expressed in fetal ovarian germ cells and might play an important role in male and female germ cell biology.
癌胚/睾丸（CT）抗原因其表达模式而得名，因为它们通常存在于各种类型的肿瘤以及正常成年睾丸的生殖细胞中。通常报道成年卵巢组织CT抗原呈阴性。基于雌性与雄性性腺发育的差异，成年卵巢中最主要的CT抗原阳性睾丸生殖细胞的卵巢对应物并不常见。因此，我们用本研究小组先前制备的各种单克隆抗体（mAb），通过免疫组织化学分析了胎儿卵巢中几种CT抗原的蛋白表达。所用的mAb为：MA454（MAGE-A1）、M3H67（MAGE-A3）、57B（MAGE-A4）、CT7-33（CT7/MAGE-C1）和ES121（NY-ESO-1）。所有mAb在胎儿卵巢生殖细胞中均显示出一定程度的免疫阳性。在妊娠16至23周期间，mAb M3H67、57B和CT7-33染色最为强烈。染色最普遍的细胞是卵原细胞，原始卵泡的卵母细胞仅有局灶性染色。我们得出结论，CT抗原在胎儿卵巢生殖细胞中正常表达，可能在雄性和雌性生殖细胞生物学中发挥重要作用。
