Carlson D, Beattie J H, Poulsen H D
Department of Animal Health, Welfare and Nutrition, Danish Institute of Agricultural Sciences, Research Centre Foulum, Tjele, Denmark.
J Anim Physiol Anim Nutr (Berl). 2007 Feb;91(1-2):19-28. doi: 10.1111/j.1439-0396.2006.00637.x.
The aim of the study was to determine the effect of weaning and the effect of increasing dietary zinc concentrations on the zinc and copper status of weaned piglets (study 1) and to study the effect of high concentrations of dietary zinc and/or copper on zinc and copper status of weaned piglets (study 2). Study 1 included 54 piglets (six litters of nine piglets). One piglet from every litter was killed 1 day before weaning. The remaining 48 piglets were allocated at weaning (28 days) to four dietary zinc treatments (100, 250, 1000 or 2500 ppm) and subsequently killed 1-2, 5-6 or 14-15 days after weaning. Study 2 included 48 piglets (six litters of eight piglets) allocated to four dietary treatments, consisting of low or high dietary zinc (100 or 2500 ppm) in combination with low or high dietary copper (20 or 175 ppm). All piglets in study 2 were killed 5-7 days after weaning. In both studies, the trace mineral status was assessed by zinc and copper concentrations and alkaline phosphatase (AP) activity in plasma and mucosal tissue. In study 2, lymphocyte metallothionein (MT) mRNA and intestinal mucosa MT mRNA concentrations were included as zinc status markers. The results showed that the zinc status, measured as zinc in plasma and mucosa, was not affected by weaning of the piglets. Plasma copper concentrations decreased during the first 2 weeks after weaning. High dietary copper concentrations did not affect the concentration of copper in plasma, but increased the concentration of copper in mucosa and the concentration of zinc in plasma. The dietary zinc treatments increased the zinc concentration in plasma as well as the zinc and MT mRNA concentration in mucosa. Lymphocyte MT mRNA concentrations did not reflect the differences in dietary zinc supplementation.
本研究的目的是确定断奶以及提高日粮锌浓度对断奶仔猪锌和铜状态的影响（研究1），并研究高浓度日粮锌和/或铜对断奶仔猪锌和铜状态的影响（研究2）。研究1包括54头仔猪（6窝，每窝9头）。每窝中有1头仔猪在断奶前1天宰杀。其余48头仔猪在断奶时（28日龄）被分配到4种日粮锌处理组（100、250、1000或2500 ppm），随后在断奶后1 - 2天、5 - 6天或14 - 15天宰杀。研究2包括48头仔猪（6窝，每窝8头），被分配到4种日粮处理组，由低或高日粮锌（100或2500 ppm）与低或高日粮铜（20或175 ppm）组合而成。研究2中的所有仔猪在断奶后5 - 7天宰杀。在两项研究中，通过血浆和黏膜组织中的锌和铜浓度以及碱性磷酸酶（AP）活性来评估微量矿物质状态。在研究2中，淋巴细胞金属硫蛋白（MT）mRNA和肠黏膜MT mRNA浓度被纳入作为锌状态标志物。结果表明，以血浆和黏膜中的锌衡量的锌状态不受仔猪断奶的影响。断奶后前2周血浆铜浓度下降。高日粮铜浓度不影响血浆中铜的浓度，但增加了黏膜中铜的浓度以及血浆中锌的浓度。日粮锌处理提高了血浆中锌的浓度以及黏膜中锌和MT mRNA的浓度。淋巴细胞MT mRNA浓度未反映日粮锌补充量的差异。
