Barba-Vidal Emili, Buttow Roll Victor Fernando, Garcia Manzanilla Edgar, Torrente Carlos, Moreno Muñoz José Antonio, Pérez José Francisco, Martín-Orúe Susana María
Animal Nutrition and Welfare Service, Departament de Ciència Animal i dels Aliments, Universitat Autònoma de Barcelona; Bellaterra, Spain.
Federal University of Pelotas, Faculty of Agronomy Eliseu Maciel, Department of Animal Science; Pelotas, RS, Brazil.
PLoS One. 2017 Oct 26;12(10):e0186781. doi: 10.1371/journal.pone.0186781. eCollection 2017.
The weaning pig is used as an experimental model to assess the impact of diet on intestinal health. Blood parameters (BP) are considered a useful tool in humans, but there is very scarce information of such indicators in the weaning pig. The objective of the present study is to evaluate the use of different BP as indicators in an experimental model of salmonellosis.
Seventy-two 28-day-old piglets were divided into four groups in a 2x2 factorial arrangement, with animals receiving or not a probiotic combination based on B. infantis IM1® and B. lactis BPL6 (109 colony forming units (cfu)/d) and orally challenged or not a week later with Salmonella Typhimurium (5x108 cfu). Blood samples of one animal per pen (N = 24) were taken four days post-inoculation for the evaluation of different BP using an I-stat® System and of plasmatic concentrations of zinc, iron and copper.
Results reported marginal deficiencies of zinc in piglets at weaning. Moreover, plasmatic zinc, copper and iron presented good correlations with weight gain (r 0.57, r -0.67, r 0.54 respectively; P < 0.01). Blood electrolytes (Na+, Cl- and K+) decreased (P < 0.01) only when the performance of the animals was seriously compromised and clinical symptoms were more apparent. Acid-base balance parameters such as HCO3-, TCO2 and BEecf significantly correlated with weight gain, but only in the challenged animals (r -0.54, r -0.55, and r -0.51, respectively; P < 0.05), suggesting metabolic acidosis depending on Salmonella infection. Glucose was affected by the challenge (P = 0.040), while Htc and Hgb increased with the challenge and decreased with the probiotic (P < 0.05). Furthermore, correlations of Glu, Htc and Hgb with weight gain were observed (P < 0.05). Overall, BP could be regarded as simple, useful indexes to assess performance and health of weaning piglets.
断奶仔猪被用作评估日粮对肠道健康影响的实验模型。血液参数（BP）在人类中被认为是一种有用的工具，但在断奶仔猪中关于此类指标的信息非常稀少。本研究的目的是评估不同血液参数作为沙门氏菌病实验模型指标的用途。
将72头28日龄仔猪按2×2析因设计分为四组，动物接受或不接受基于婴儿双歧杆菌IM1®和乳酸双歧杆菌BPL6（109菌落形成单位（cfu）/天）的益生菌组合，并在一周后口服或不口服鼠伤寒沙门氏菌（5×108 cfu）。接种后四天，从每栏选取一头动物（N = 24）采集血样，使用I-stat®系统评估不同血液参数以及血浆锌、铁和铜的浓度。
结果表明断奶仔猪存在边缘性锌缺乏。此外，血浆锌、铜和铁与体重增加呈现良好的相关性（分别为r = 0.57、r = -0.67、r = 0.54；P < 0.01）。仅当动物的生长性能严重受损且临床症状更明显时，血液电解质（Na +、Cl - 和K +）才会降低（P < 0.01）。酸碱平衡参数如HCO3 -、TCO2和BEecf与体重增加显著相关，但仅在受挑战的动物中如此（分别为r = -0.54、r = -0.55和r = -0.51；P < 0.05），提示取决于沙门氏菌感染的代谢性酸中毒。葡萄糖受挑战影响（P = 0.040），而血细胞比容（Htc）和血红蛋白（Hgb）随挑战增加而升高，随益生菌使用而降低（P < 0.05）。此外，观察到Glu、Htc和Hgb与体重增加之间的相关性（P < 0.05）。总体而言，血液参数可被视为评估断奶仔猪生长性能和健康状况的简单且有用的指标。
