Cahill Leah E, Peng Claudia Yu-Chen, Bankovic-Calic Neda, Sankaran Deepa, Ogborn Malcolm R, Aukema Harold M
Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, Canada.
Br J Nutr. 2007 Jan;97(1):77-84. doi: 10.1017/S0007114507250470.
Dietary soya protein substitution for casein initiated at weaning slows disease progression in animal models of chronic renal disease. As there is increasing evidence that fetal programming can have a significant impact on kidney physiology and function in offspring, the objective of the current study was to determine whether exposure to soya protein in the diet earlier than weaning would have further benefits. Han:SPRD-cy (cy/+) breeder rats were fed a casein-based or soya protein-based diet 2 weeks prior to mating, throughout pregnancy and during lactation. Following this maternal period, 3-week-old pups were given either the same or the alternate diet for a 7-week weaning period. Dietary soya protein compared with casein in the maternal or weaning period both independently resulted in less renal inflammation (macrophage infiltration lower by 24% (P=0.0003) and 32% (P<0.001), respectively). When soya protein was given in both feeding periods, the effect was additive. Soya protein substitution for casein resulted in less oxidative damages as indicated by 28% lower oxidized-LDL staining (P=0.013) when present in the maternal period, or in the weaning period (by 56%, P<0.0001). Renal cell proliferation was reduced by 29-33% (P<0.05) in rats given soya protein whether the exposure was during the maternal or weaning period. Soya protein compared with casein in the maternal period also resulted in 33% (P=0.0013) less proteinuria, indicating superior renal function. Dietary soya protein during pregnancy and lactation represents a potential preventative approach in treating for those with congenital kidney diseases.
断奶时开始用膳食大豆蛋白替代酪蛋白可减缓慢性肾病动物模型中的疾病进展。由于越来越多的证据表明胎儿编程会对后代的肾脏生理和功能产生重大影响，本研究的目的是确定在断奶前更早地接触膳食中的大豆蛋白是否会有更多益处。将Han:SPRD-cy (cy/+) 繁殖大鼠在交配前2周、整个孕期和哺乳期喂食基于酪蛋白或大豆蛋白的饮食。在这段母鼠喂养期之后，给3周龄的幼崽在7周的断奶期喂食相同或替代饮食。在母鼠期或断奶期，膳食大豆蛋白与酪蛋白相比，均独立导致肾脏炎症减轻（巨噬细胞浸润分别降低24%（P = 0.0003）和32%（P < 0.001））。当在两个喂养期都给予大豆蛋白时，效果是相加的。大豆蛋白替代酪蛋白导致氧化损伤减少，如在母鼠期或断奶期存在时，氧化型低密度脂蛋白染色降低28%（P = 0.013）或56%（P < 0.0001）所示。无论在母鼠期还是断奶期接触大豆蛋白，大鼠的肾细胞增殖均减少29 - 33%（P < 0.05）。在母鼠期，大豆蛋白与酪蛋白相比还导致蛋白尿减少33%（P = 0.0013），表明肾功能更优。孕期和哺乳期的膳食大豆蛋白是治疗先天性肾病患者的一种潜在预防方法。
