Castritsi-Catharios J, Bourdaniotis N, Persoone G
National and Kapodistrian University of Athens, Department of Biology, Sector of Zoology and Marine Biology, University Campus, Athens 157 84, Greece.
Chemosphere. 2007 Apr;67(6):1127-32. doi: 10.1016/j.chemosphere.2006.11.033. Epub 2007 Jan 10.
The use of antifouling paints is the only truly effective method for the protection of underwater structures from the development of fouling organisms. In the present study, the surface to volume concept constitutes the basis for the development of a new and improved method for determining the toxicity of antifouling paints on marine organisms. Particular emphasis is placed on the attainment of a standardized uniformity of coated surfaces. Failure to control the thickness of the coat of paint in previous studies of this type, has led to inaccurate evaluation of the relative toxicity of samples. Herein, an attempt is made to solve this problem using a simple technique which gives completely uniform and smooth surfaces. The effectiveness of this technique is assessed through two series of experiments using two different types of test containers: 50 ml modified syringes and 7 ml multiwells. The results of the toxicity experiments follow a normal distribution around the average value which allows to consider these values as reliable for comparison of the level of toxic effect detected with the two types of test containers. The mean lethal concentration L(S/V)(50) in the test series conducted in the multiwells (20.38 mm(2)ml(-1)) does not differ significantly from that obtained in the test series using modified syringes (20.065 mm(2)ml(-1)). It can thus be concluded from this preliminary study that the new method and the two different ways of exposing the test organisms to the antifouling paints and their leachates gave reliable and replicable results.
使用防污涂料是保护水下结构免受污损生物侵害的唯一真正有效方法。在本研究中，表面与体积概念构成了开发一种新的、改进的测定防污涂料对海洋生物毒性方法的基础。特别强调实现涂层表面的标准化均匀性。在以往此类研究中，未能控制涂料涂层的厚度导致对样品相对毒性的评估不准确。在此，尝试使用一种能产生完全均匀且光滑表面的简单技术来解决这个问题。通过使用两种不同类型的测试容器（50毫升改良注射器和7毫升多孔板）进行的两个系列实验来评估该技术的有效性。毒性实验结果围绕平均值呈正态分布，这使得可以认为这些值对于比较两种测试容器检测到的毒性效应水平是可靠的。在多孔板中进行的测试系列的平均致死浓度L(S/V)(50)（20.38平方毫米毫升⁻¹）与使用改良注射器的测试系列中获得的结果（20.065平方毫米毫升⁻¹）没有显著差异。因此，从这项初步研究可以得出结论，新方法以及将测试生物暴露于防污涂料及其沥滤液的两种不同方式给出了可靠且可重复的结果。
