Department of Biotechnology, School of Environmental and Chemical Engineering, Dalian Jiaotong University, Dalian, Liaoning, P. R. China.
Toxicol Mech Methods. 2012 Jan;22(1):23-30. doi: 10.3109/15376516.2011.583297. Epub 2011 Aug 23.
This article described an improved brine shrimp larvae lethality microwell test method. A simply designed connecting vessel with alternative photoperiod was used to culture and collect high yield of active Artemia parthenogenetica nauplii for brine shrimp larvae lethality microwell test. Using this method, pure A. parthenogenetica nauplii suspension was easily cultured and harvested with high density about 100-150 larvae per milliliter and the natural mortality was reduced to near zero by elimination of unnecessary artificial disturbance. And its sensitivity was validated by determination of LC(50)-24 h of different reference toxicants including five antitumor agents, two pesticides, three organic pollutants, and four heavy metals salts, most of which exhibited LC(50)-24 h between 0.07 and 58.43 mg/L except for bleomycin and mitomycin C with LC(50)-24 h over 300 mg/L.
本文描述了一种改良的丰年虾幼虫致死性微量测试方法。使用一种简单设计的带有交替光周期的连接容器来培养和收集高产的活卤虫无节幼体，用于丰年虾幼虫致死性微量测试。使用这种方法，很容易培养和收获纯卤虫无节幼体悬浮液，密度高达每毫升 100-150 个幼虫，并且通过消除不必要的人为干扰，将自然死亡率降低到接近零。通过测定包括五种抗肿瘤剂、两种农药、三种有机污染物和四种重金属盐在内的不同参考毒物的 LC(50)-24 h，验证了其灵敏度，除博莱霉素和丝裂霉素 C 的 LC(50)-24 h 超过 300 mg/L 外，大多数毒物的 LC(50)-24 h 介于 0.07 和 58.43 mg/L 之间。
