Kim Namju, Kunisawa Jun, Kweon Mi-Na, Eog Ji Geun, Kiyono Hiroshi
Department of Food and Nutrition, Seoul National University, Seoul, South Korea.
Clin Immunol. 2007 Apr;123(1):30-9. doi: 10.1016/j.clim.2006.11.005. Epub 2007 Jan 10.
Probiotics have been considered as preventive agents for the control of inflammatory bowel disease (IBD). In this study, we assessed the immunomodulatory effect of Bifidobacterium bifidum BGN4 on the control of IBD using the CD4(+) CD45RB(high) T cell transfer disease model. The mice were fed for 4 weeks with either a conventional diet containing only skim milk or a diet containing skim milk with 0.3% (w/w) BGN4. The BGN4-fed mice showed normal weight growth, fewer clinical symptoms such as thickened wall and inflammatory cell infiltration, and lower levels of CD4(+) T lymphocyte infiltration and inflammatory cytokine productions than the skim milk-fed mice with IBD in the large intestine. Suppression of these cytokine productions, particularly IFN-gamma and MCP-1, through BGN4 treatment was also observed in the in vitro co-culture between intestinal epithelial cells and T cells. These findings suggested that a BGN4 supplemented diet could be helpful for the control of aberrant immune responses in the intestinal tissue.
益生菌已被视为控制炎症性肠病（IBD）的预防剂。在本研究中，我们使用CD4(+) CD45RB(high) T细胞转移疾病模型评估了双歧双歧杆菌BGN4对IBD控制的免疫调节作用。将小鼠用仅含脱脂牛奶的常规饮食或含0.3%（w/w）BGN4的脱脂牛奶饮食喂养4周。与患有IBD的喂食脱脂牛奶的小鼠相比，喂食BGN4的小鼠体重正常增长，临床症状如肠壁增厚和炎性细胞浸润较少，大肠中CD4(+) T淋巴细胞浸润水平和炎性细胞因子产生较低。在肠上皮细胞和T细胞之间的体外共培养中也观察到通过BGN4处理对这些细胞因子产生的抑制作用，特别是IFN-γ和MCP-1。这些发现表明，补充BGN4的饮食可能有助于控制肠道组织中异常的免疫反应。
