Lee Nayeon, Lee Soomin, Jang Se-Won, Shin Hee Soon, Park Jong-Hyun, Park Myeong Soo, Lee Byung-Hoo
Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of Korea.
Korea Food Research Institute, Wanju 55365, Republic of Korea.
Saudi J Biol Sci. 2021 Sep;28(9):5115-5118. doi: 10.1016/j.sjbs.2021.05.028. Epub 2021 May 20.
BGN4 has been shown to improve the immune system by regulating interleukin (IL)-6 in RAW 264.7 macrophage cells. In this study, the dead cells of BGN4 were produced by enzymatic and physical processing to enhance the inhibition properties of pro-inflammatory cytokines using lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Notably, the secretion levels of cytokines such as interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α were decreased by the cell-wall disrupted extracts compared to heat-killed cells. The result suggests that the exposed interior-surface of BGN4 has a potential ability to regulate the immune-responses in the gastrointestinal tract due to major substances in inside-cell wall such as peptidoglycan and teichoic acids. In conclusion, the lysed and disrupted cells from the inside out of BGN4 have anti-inflammatory properties as paraprobiotic agents to control chronic inflammatory related-diseases.
已证明BGN4可通过调节RAW 264.7巨噬细胞中的白细胞介素（IL）-6来改善免疫系统。在本研究中，通过酶促和物理处理产生BGN4的死细胞，以增强使用脂多糖（LPS）刺激的RAW 264.7细胞对促炎细胞因子的抑制特性。值得注意的是，与热杀死的细胞相比，细胞壁破坏提取物降低了白细胞介素（IL）-1β、IL-6、IL-8和肿瘤坏死因子（TNF）-α等细胞因子的分泌水平。结果表明，由于细胞壁内部的主要物质如肽聚糖和磷壁酸，BGN4暴露的内表面具有调节胃肠道免疫反应的潜在能力。总之，从内到外裂解和破坏的BGN4细胞具有作为副益生菌控制慢性炎症相关疾病的抗炎特性。
