O'Neill G P, Grygorczyk R, Adam M, Ford-Hutchinson A W
Department of Pharmacology, Merck-Frosst Centre for Therapeutic Research, Kirkland, Canada.
Biochim Biophys Acta. 1991 Dec 2;1129(1):131-4. doi: 10.1016/0167-4781(91)90228-e.
The cDNA encoding the voltage-gated chloride channel from the electric organ of Torpedo californica has been isolated and sequenced. The 2.7 kilobase pair cDNA encodes an 810 amino acid polypeptide which is highly homologous at both the DNA (97%) and amino acid (97%) levels to the voltage-gated chloride channel from the electric organ of T. marmorata. The majority of the 24 amino acid differences between the T. californica and T. marmorata voltage-gated chloride channels are clustered in two putative cytoplasmic domains with six differences located between residues 10-92 and 14 differences occurring between residues 576 to 708. Only one amino acid difference occurs in one of the predicted transmembrane domains. The most dramatic difference is an insertion of Asp-Val-Pro-Gly in a large cytoplasmic domain at amino acid residue 627 of the T. californica channel.
编码加州电鳐电器官电压门控氯离子通道的cDNA已被分离并测序。这个2.7千碱基对的cDNA编码一个810个氨基酸的多肽，该多肽在DNA水平（97%）和氨基酸水平（97%）上与斑纹电鳐电器官的电压门控氯离子通道高度同源。加州电鳐和斑纹电鳐电压门控氯离子通道之间的24个氨基酸差异中的大多数集中在两个假定的胞质结构域中，其中6个差异位于第10至92位残基之间，14个差异出现在第576至708位残基之间。在一个预测的跨膜结构域中仅出现一个氨基酸差异。最显著的差异是在加州电鳐通道第627位氨基酸残基处的一个大的胞质结构域中有天冬氨酸-缬氨酸-脯氨酸-甘氨酸的插入。
