通过在非洲爪蟾卵母细胞中进行表达克隆分离出的电鳐氯化物通道的一级结构。

Primary structure of Torpedo marmorata chloride channel isolated by expression cloning in Xenopus oocytes.

作者信息

Jentsch T J, Steinmeyer K, Schwarz G

机构信息

Centre for Molecular Neurobiology (ZMNH), Hamburg University, Germany.

出版信息

Nature. 1990 Dec 6;348(6301):510-4. doi: 10.1038/348510a0.

DOI:10.1038/348510a0

PMID:2174129

Abstract

A complementary DNA encoding a voltage-gated chloride channel from Torpedo marmorata electric organ was cloned by expressing hybrid-depleted messenger RNA in Xenopus oocytes. The predicted protein has a sequence of 805 amino acids containing several putative membrane-spanning domains. Expression of the protein in Xenopus oocytes shows that it is sufficient for channel function.

摘要

通过在非洲爪蟾卵母细胞中表达杂交缺失信使核糖核酸，克隆了一种来自电鳐电器官的编码电压门控氯离子通道的互补脱氧核糖核酸。预测的蛋白质有805个氨基酸序列，包含几个假定的跨膜结构域。该蛋白质在非洲爪蟾卵母细胞中的表达表明它足以实现通道功能。