Raveche E S, Tjio J H
Department of Pathology, UMDNJ, New Jersey Medical School, Newark 07103.
Int J Hematol. 1991 Feb;54(1):25-35.
Hyperdiploid B cells have been found in autoimmune NZB mice as they age. The hyperdiploid cells were found to be clonal both on the basis of cytogenetic analysis and studies of immunoglobulin gene rearrangements at the DNA level. Studies of the inheritance of the hyperdiploid traits in both F1 and backcrosses, as well as NZB recombinant inbred strains, revealed that the presence of hyperdiploid B cells was an inherited recessive trait linked to autoimmune hyperactivity. In addition, hyperdiploid B cells were found to possess a unique chromosome pair which lacked terminal C-bands. This observation allowed analysis of the fate of transferred NZB hyperdiploid B cells into unirradiated recipients. The hyperdiploid B cells were found to expand in recipients and become the dominant population in several lymphoid organs. Spontaneously occurring hyperdiploid B cells were not observed in NZB-xid mice possessing the CBA/N X chromosome, which confers abnormal B cell maturation and results in decreased autoimmunity in NZB-xid mice. Following the discovery that CD5+ B cells were elevated in certain autoimmune states, hyperdiploid B cells were examined and found to be CD5+ B cells as well. The malignant cell in chronic lymphocytic leukemia is also a CD5+ B cell. The hyperdiploid B cells of NZB mice appear to have many of the features of autoimmune B cells, as well as malignant cells.
随着年龄增长，在自身免疫性NZB小鼠中发现了超二倍体B细胞。基于细胞遗传学分析以及DNA水平上免疫球蛋白基因重排的研究，发现这些超二倍体细胞是克隆性的。对F1代、回交后代以及NZB重组近交系中超二倍体特征遗传情况的研究表明，超二倍体B细胞的存在是一种与自身免疫性亢进相关的隐性遗传性状。此外，发现超二倍体B细胞拥有一对独特的染色体，其缺乏末端C带。这一观察结果使得对转移至未受照射受体体内的NZB超二倍体B细胞的命运进行分析成为可能。结果发现，超二倍体B细胞在受体中扩增，并成为多个淋巴器官中的优势细胞群体。在具有CBA/N X染色体、导致B细胞成熟异常并使NZB-xid小鼠自身免疫性降低的NZB-xid小鼠中，未观察到自发出现的超二倍体B细胞。在发现某些自身免疫状态下CD5+B细胞数量增加之后，对超二倍体B细胞进行检查，发现它们也是CD5+B细胞。慢性淋巴细胞白血病中的恶性细胞也是CD5+B细胞。NZB小鼠的超二倍体B细胞似乎具有自身免疫性B细胞以及恶性细胞的许多特征。
