Raveche E S, Lalor P, Stall A, Conroy J
Department of Microbiology and Immunology, Albany Medical College, NY 12208.
J Immunol. 1988 Dec 15;141(12):4133-9.
Cells with increased chromosome number and DNA content have been found in the spleens of old NZB mice. These hyperdiploid cells are of clonal origin and demonstrate discrete IgH chain gene rearrangements by Southern blot analysis. In this report, hyperdiploid cells were analyzed by three-color flow cytometric techniques and found to be Ly-1+ B cells which were dull for Ly-1 and bright for surface IgM. These cells, unlike typical diploid Ly-1+ B cells, were negative for B220/6B2 and surface IgD. Hyperdiploid Ly-1+ B cells were found to be the predominant splenic subpopulation in animals receiving a spleen cell transfer from donors which possessed hyperdiploid Ly-1+ B cells. (NZB x DBA/2)F1 recipients of NZB spleen cells demonstrated a 10- to 1000-fold increase in Ly-1+ B cells in the spleen but showed no increased levels of Ly-1+ B cells in the peritoneum. Nearly all the splenic Ly-1+ B cells were hyperdiploid with the phenotype of the NZB parent. Cytogenetic analysis revealed that all the hyperdiploid cells were NZB donor cells. These findings suggest that the increase in splenic Ly-1+ B cells in the F1 recipients was due to expansion of injected splenic hyperdiploid Ly-1+ B cells of NZB origin. All of the F1 recipients of NZB hyperdiploid Ly-1+ B cells demonstrated a significant decrease in endogenous B cells as well as decreased serum IgM and anti-ssDNA autoantibodies. These studies suggest that hyperdiploid Ly-1+ B cells are different from typical peritoneal Ly-1+ B cells both in the lymphoid organs to which they home and in their proliferative capacity. NZB hyperdiploid Ly-1+ B cells, which may arise as a natural consequence of hyperactive Ly-1+ B cells, may play an immunoregulatory role in the spleen.
在老龄新西兰黑鼠（NZB）的脾脏中发现了染色体数目和DNA含量增加的细胞。这些超二倍体细胞起源于克隆，通过Southern印迹分析显示出离散的免疫球蛋白重链（IgH）基因重排。在本报告中，采用三色流式细胞术对超二倍体细胞进行分析，发现它们是Ly-1+B细胞，Ly-1表达暗淡而表面免疫球蛋白M（IgM）表达明亮。这些细胞与典型的二倍体Ly-1+B细胞不同，B220/6B2和表面免疫球蛋白D（IgD）呈阴性。在接受来自具有超二倍体Ly-1+B细胞供体的脾细胞转移的动物中，发现超二倍体Ly-1+B细胞是主要的脾脏亚群。接受NZB脾细胞的（NZB×DBA/2）F1代受体脾脏中Ly-1+B细胞增加了10到1000倍，但腹膜中Ly-1+B细胞水平没有升高。几乎所有脾脏Ly-1+B细胞都是超二倍体，具有NZB亲本的表型。细胞遗传学分析表明，所有超二倍体细胞都是NZB供体细胞。这些发现表明，F1代受体脾脏中Ly-1+B细胞的增加是由于注射的源自NZB的脾脏超二倍体Ly-1+B细胞的扩增。所有接受NZB超二倍体Ly-1+B细胞的F1代受体的内源性B细胞均显著减少，血清IgM和抗单链DNA自身抗体也减少。这些研究表明，超二倍体Ly-1+B细胞在其归巢的淋巴器官及其增殖能力方面均与典型的腹膜Ly-1+B细胞不同。NZB超二倍体Ly-1+B细胞可能是Ly-1+B细胞过度活跃的自然结果，可能在脾脏中发挥免疫调节作用。
