非洲爪蟾c-raf原癌基因：卵子发生和早期发育过程中的克隆与表达

Xenopus c-raf proto-oncogene: cloning and expression during oogenesis and early development.

作者信息

Le Guellec R, Couturier A, Le Guellec K, Paris J, Le Fur N, Philippe M

机构信息

CNRS URA 256, Université de Rennes I, France.

出版信息

Biol Cell. 1991;72(1-2):39-45. doi: 10.1016/0248-4900(91)90076-y.

DOI:10.1016/0248-4900(91)90076-y

PMID:1721855

Abstract

We have isolated and characterized a cDNA which contains the entire coding sequence of Xenopus laevis raf protein. raf mRNA is identified as a member of the class of maternal RNAs. It is already relatively abundant at the beginning of oogenesis and is stable at least until the midblastula transition. The RNA is also detected later during embryogenesis in particular in gastrula, neurula, tailbud and feeding tadpole. We have also found the RNA in several adult tissues (skin, testis, stomach, intestine) at different levels.

摘要

我们已经分离并鉴定了一个包含非洲爪蟾raf蛋白完整编码序列的cDNA。raf mRNA被鉴定为母源RNA类别的成员。它在卵子发生开始时就已经相对丰富，并且至少在囊胚中期转变之前是稳定的。在胚胎发育后期，特别是在原肠胚、神经胚、尾芽和摄食蝌蚪中也能检测到这种RNA。我们还在几种成年组织（皮肤、睾丸、胃、肠道）中发现了不同水平的这种RNA。