Javaheri Michael, Fujii Gildo Y, Rossi Juliana V, Panzan Carla Q, Yanai Douglas, Lakhanpal Rohit R, Maia Mauricio, Khurana Rahul N, Guven Dilek, De Juan Eugene, Humayun Mark S
Doheny Retina Institute, Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, California 90033, USA.
Retina. 2007 Jan;27(1):87-94. doi: 10.1097/01.iae.0000256665.79487.a4.
To investigate the effect of oxygenated intraocular irrigating solutions on electroretinograms (ERGs) for postvitrectomy rabbits.
Eight groups of five rabbits each underwent pars plana vitrectomy on the right eye; the left eye of each rabbit served as control. The intraocular irrigating solutions were balanced salt solution (BSS), BSS-plus, BSS + oxygen (BSS + O2), BSS-plus + O2, and combinations of each with the addition of endoillumination (L). All animals were evaluated by single-flash scotopic electroretinography on the operated and nonoperated eyes before surgery and at 1 hour, 1 day, 1 week, and 1 month after surgery by an unmasked observer. The main outcome measures were dark-adapted b-wave amplitudes of operated eye/nonoperated eye ratios. The results for the different groups were compared by analysis of variance.
Mean dark-adapted b-wave amplitudes of operated eye/nonoperated eye ratios +/- SD for BSS-plus and BSS-plus + O2 before surgery and 1 hour, 1 day, 1 week, and 1 month after surgery were 1.01 +/- 0.09, 0.50 +/- 0.11, 0.95 +/- 0.11, 0.97 +/- 0.11, and 0.99 +/- 0.08 and 0.98 +/- 0.08, 0.59 +/- 0.10, 0.92 +/- 0.06, 0.97 +/- 0.08, and 0.97 +/- 0.10, respectively. At both 1 hour and 1 day after surgery, rabbits treated with BSS-plus + O2 had an earlier b-wave return to baseline findings, but the differences were not statistically significant (P > 0.05). Mean dark-adapted b-wave amplitudes of operated eye/nonoperated eye ratios +/- SD for BSS and BSS + O2 before surgery and 1 hour, 1 day, 1 week, and 1 month after surgery were 1.02 +/- 0.10, 0.47 +/- 0.09, 0.77 +/- 0.07, 0.89 +/- 0.07, and 0.89 +/- 0.07 and 1.02 +/- 0.06, 0.62 +/- 0.16, 0.94 +/- 0.09, 0.97 +/- 0.08, and 0.96 +/- 0.06, respectively. One hour and 1 day after surgery, ERG readings for rabbits treated with BSS + O2 exhibited a statistically significantly earlier return of ERG voltage to baseline values compared with both BSS and BSS + L (P = 0.05 and P = 0.02, respectively). One day after surgery, rabbits treated with BSS alone had the lowest ERG ratios. One week and 1 month after surgery, for all solutions tested other than BSS, ERG values were within normal limits.
The use of oxygenated solutions appears to affect ERG readings after pars plana vitrectomy. Further studies to evaluate the role of oxygenated solutions in different vitreoretinal surgical procedures are warranted.
研究含氧眼内冲洗液对玻璃体切除术后兔视网膜电图（ERG）的影响。
八组实验，每组五只兔，右眼行扁平部玻璃体切除术；每只兔的左眼作为对照。眼内冲洗液为平衡盐溶液（BSS）、BSS-plus、BSS + 氧气（BSS + O2）、BSS-plus + O2，以及每种溶液添加眼内照明（L）的组合。所有动物在手术前、手术后1小时、1天、1周和1个月，由未戴眼罩的观察者对手术眼和未手术眼进行单次闪光暗视视网膜电图评估。主要观察指标为手术眼/未手术眼的暗适应b波振幅比值。不同组的结果采用方差分析进行比较。
BSS-plus和BSS-plus + O2在手术前、手术后1小时、1天、1周和1个月的手术眼/未手术眼暗适应b波平均振幅比值±标准差分别为1.01±0.09、0.50±0.11、0.95±0.11、0.97±0.11和0.99±0.08，以及0.98±0.08、0.59±0.10、0.92±0.06、0.97±0.08和0.97±0.10。在手术后1小时和1天，接受BSS-plus + O2治疗的兔b波恢复到基线值的时间较早，但差异无统计学意义（P>0.05）。BSS和BSS + O2在手术前、手术后1小时、1天、1周和1个月的手术眼/未手术眼暗适应b波平均振幅比值±标准差分别为1.02±0.10、0.47±0.09、0.77±0.07、0.89±0.07和0.89±0.07，以及1.02±0.06、0.62±0.16、0.94±0.09、0.97±0.08和0.96±0.06。手术后1小时和1天，接受BSS + O2治疗的兔ERG读数显示，与BSS和BSS + L相比，ERG电压恢复到基线值的时间在统计学上显著更早（分别为P = 0.05和P = 0.02）。手术后1天，仅接受BSS治疗的兔ERG比值最低。手术后1周和1个月，除BSS外的所有测试溶液的ERG值均在正常范围内。
使用含氧溶液似乎会影响扁平部玻璃体切除术后的ERG读数。有必要进一步研究评估含氧溶液在不同玻璃体视网膜手术中的作用。
