Sae-be Porntip, Sangwatanaroj Usa, Punnapayak Hunsa
Department of Materials Science, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Biotechnol J. 2007 Mar;2(3):316-25. doi: 10.1002/biot.200600080.
This article discusses the analysis of the hydrolysis products from one-step scouring of cotton using pectinase and two-step scouring of cotton using lipase then cellulase, protease then cellulase, or lipase/protease then cellulase, to improve water absorbency of cotton. UV spectrophotometric analysis indicated that the pectinase scouring process produced approximately 18-fold higher amounts of reducing sugars and galacturonic acid than any of the two-step scouring processes. The production rate of reducing sugars and galacturonic acid from most of the scouring processes showed a decrease with an increase in time. HPLC analysis revealed that the lipase/protease/cellulase scouring processes produced approximately 5-fold higher amounts of 17 amino acids than the pectinase scouring process. GC analysis for 18 fatty acids (C(8)-C(24)) revealed that three major fatty acids, palmitic acid, stearic acid, and behenic acid, were found on both the scoured and the unscoured fabrics. Scoured fabrics were tested for content of proteins, extractable components, waxes, and anionic components including pectins, and some differences among the fabric scoured with different enzyme combinations were found.
本文讨论了使用果胶酶对棉织物进行一步精练以及使用脂肪酶然后纤维素酶、蛋白酶然后纤维素酶或脂肪酶/蛋白酶然后纤维素酶对棉织物进行两步精练后水解产物的分析，以提高棉织物的吸水性。紫外分光光度分析表明，果胶酶精练过程产生的还原糖和半乳糖醛酸量比任何两步精练过程高出约18倍。大多数精练过程中还原糖和半乳糖醛酸的生成速率随时间增加而降低。高效液相色谱分析显示，脂肪酶/蛋白酶/纤维素酶精练过程产生的17种氨基酸量比果胶酶精练过程高出约5倍。对18种脂肪酸（C(8)-C(24)）的气相色谱分析表明，在精练和未精练的织物上均发现了三种主要脂肪酸，即棕榈酸、硬脂酸和山嵛酸。对精练织物的蛋白质、可提取物成分、蜡以及包括果胶在内的阴离子成分含量进行了测试，发现不同酶组合精练的织物之间存在一些差异。
