Suzuki K, Sakata N, Hara M, Kitani A, Harigai M, Hirose W, Kawaguchi Y, Kawagoe M, Nakamura H
Internal Medicine I, National Defense Medical College, Saitama, Japan.
Biochem Cell Biol. 1991 Aug;69(8):571-6. doi: 10.1139/o91-085.
Active uptake of a free fatty acid, oleate, and its incorporation into triacylglycerol and phospholipid in the human monocytic cell line, U937, was identified using 14C-labelled oleate. Excess triacylglycerol accumulates in the form of lipid droplets in the cytoplasm. The extent of lipid droplet formation in each cell can be assessed in the absence of any staining by 90 degrees light scatter intensity, one of the basic parameters of flow cytometry.
利用14C标记的油酸，确定了人单核细胞系U937对游离脂肪酸油酸的主动摄取及其掺入三酰甘油和磷脂的过程。过量的三酰甘油以脂滴的形式积聚在细胞质中。每个细胞中脂滴形成的程度可以在不进行任何染色的情况下，通过90度光散射强度来评估，这是流式细胞术的基本参数之一。
