Szamocki Rafael, Velichko Alexandra, Holzapfel Christian, Mücklich Frank, Ravaine Serge, Garrigue Patrick, Sojic Neso, Hempelmann Rolf, Kuhn Alexander
Laboratoire d'Analyse Chimique par Reconnaissance Moléculaire, ENSCPB, 16 avenue Pey Berland, 33607 Pessac, France.
Anal Chem. 2007 Jan 15;79(2):533-9. doi: 10.1021/ac0615854.
Recent work on the preparation of highly organized macroporous electrodes and nanoporous ultramicroelectrodes has been combined and extended to elaborate macroporous ultramicroelectrodes (UMEs) by template synthesis using colloidal crystals and following two different and complementary methods. On the one hand, arched porous UMEs were prepared, and on the other hand, cylindrical porous UMEs were obtained by using cavity UMEs. These macroporous UMEs have an active surface area which is up to 2 orders of magnitude higher compared to that of a classical disk UME as characterized by cyclic voltammetry. To study their analytical performance, the macroporous UMEs have been modified with a redox-active thiol and also a model bioelectrocatalytical system containing a redox mediator, a cofactor, and glucose-dehydrogenase. In both cases the electrochemical signal is amplified by up to 2 orders of magnitude, which increases significantly the analytical performance of such electrodes and therefore opens up new applications for this kind of miniaturized electrochemical system.
近期关于制备高度有序大孔电极和纳米孔超微电极的工作已结合并扩展，通过使用胶体晶体的模板合成以及两种不同且互补的方法来精心制备大孔超微电极（UME）。一方面，制备了拱形多孔UME，另一方面，通过使用腔型UME获得了圆柱形多孔UME。这些大孔UME具有的活性表面积，与通过循环伏安法表征的经典圆盘UME相比，高出多达2个数量级。为研究其分析性能，大孔UME已用氧化还原活性硫醇以及包含氧化还原介质、辅因子和葡萄糖脱氢酶的模型生物电催化系统进行了修饰。在这两种情况下，电化学信号均被放大多达2个数量级，这显著提高了此类电极的分析性能，从而为这种小型化电化学系统开辟了新的应用领域。
