采用可重复使用的溶胶-凝胶免疫亲和柱进行选择性样品净化，用于测定食品和饲料样品中的脱氧雪腐镰刀菌烯醇。
Selective sample cleanup by reusable sol-gel immunoaffinity columns for determination of deoxynivalenol in food and feed samples.
作者信息
Brenn-Struckhofova Zdenka, Cichna-Markl Margit, Böhm Christina, Razzazi-Fazeli Ebrahim
机构信息
Department of Analytical and Food Chemistry, University of Vienna, Währinger Strasse 38, A-1090 Vienna, Austria.
出版信息
Anal Chem. 2007 Jan 15;79(2):710-7. doi: 10.1021/ac061672w.
The paper describes the development of a simple and highly selective method for the determination of deoxynivalenol (DON) in food and feed samples. It combines sample cleanup with sol-gel immunoaffinity columns containing monoclonal anti-DON antibodies and quantification of DON by high-performance liquid chromatography with ultraviolet detection. The sol-gel immunoaffinity columns are as selective as commercial DON immunoaffinity columns but superior with regard to production costs, storage stability, and reusability. In applying the method for the analysis of maize, wheat, and spaghetti samples, it offers detection limits (LOD, S/N = 3) of 240, 200, and 207 ng/g, and recoveries of 83, 99, and 97%, respectively.
本文描述了一种用于测定食品和饲料样品中脱氧雪腐镰刀菌烯醇（DON）的简单且高选择性方法的开发。它将样品净化与含有抗DON单克隆抗体的溶胶-凝胶免疫亲和柱相结合，并通过带紫外检测的高效液相色谱法定量DON。溶胶-凝胶免疫亲和柱与市售DON免疫亲和柱一样具有选择性，但在生产成本、储存稳定性和可重复使用性方面更具优势。在将该方法应用于玉米、小麦和意大利面样品分析时，其检测限（LOD，S/N = 3）分别为240、200和207 ng/g，回收率分别为83%、99%和97%。
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