凝胶免疫亲和色谱-高效液相色谱法测定有机食品和饲料中的脱氧雪腐镰刀菌烯醇。

Determination of deoxynivalenol in organic and conventional food and feed by sol-gel immunoaffinity chromatography and HPLC-UV detection.

机构信息

Department of Analytical and Food Chemistry, University of Vienna, Austria.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2010 Jan 15;878(2):187-93. doi: 10.1016/j.jchromb.2009.08.016. Epub 2009 Aug 21.

DOI:10.1016/j.jchromb.2009.08.016

PMID:19736050

Abstract

The paper describes the determination of deoxynivalenol (DON) in 55 wheat food and feed samples, 26 from conventional and 29 from organic production. Immunoaffinity columns prepared by entrapping anti-DON antibodies by the sol-gel method were used for sample clean-up. DON was quantified by high performance liquid chromatography (HPLC) and ultraviolet (UV) detection. In general, the incidence of DON contamination was rather low. In eight samples (14.5%) the DON concentration was above the LOQ (380ng/g), in six samples (10.9%) DON was detected but could not be quantified (>LOD (200ng/g), <LOQ). In seven conventional samples (two pasta, two cookie, two snack and one feed sample) but only in one organic sample (a snack) the DON concentration was >LOQ. The data indicate both a higher incidence of DON contamination and higher DON concentrations in food and feed samples from conventional than in those from organic production.

摘要

本文描述了对 55 个小麦食品和饲料样品（26 个来自常规生产，29 个来自有机生产）中脱氧雪腐镰刀菌烯醇（DON）的测定。通过溶胶-凝胶法将抗 DON 抗体包埋在免疫亲和柱中用于样品净化。DON 通过高效液相色谱（HPLC）和紫外（UV）检测进行定量。总的来说，DON 的污染发生率相当低。在 8 个样品（14.5%）中，DON 浓度高于定量限（380ng/g），在 6 个样品（10.9%）中检测到 DON，但无法定量（>LOD（200ng/g），<LOQ）。在 7 个常规样品（2 个意大利面、2 个饼干、2 个零食和 1 个饲料样品）中，但仅在 1 个有机样品（零食）中，DON 浓度高于定量限。数据表明，常规生产的食品和饲料样品中 DON 的污染发生率和浓度均高于有机生产的样品。