Chen T T, Mora E C, Mealey J
Cancer Res. 1975 Dec;35(12):3566-70.
In vitro cultivation of medulloblastoma cells was successfully established from hamster brain tumors induced by simian adenovirus SA7. These tumor cells possess morphological features in cultures that are very similar to those of the original tumor. Both tubular and rosette growth patterns were evident. At the ultrastructural level only small numbers of cytoplasmic organelles could be detected in the tumor cells, typical of generally immature and/or undifferentiated cells. The proliferation of these tumor cells depended upon properly dense initial platings. In addition, it was found that single medulloblastoma cells could be stimulated to produce colonies if treated for 10 days with conditioned medium. Inoculation of these cells into synergenic animals resulted in 100% uptake. Survival of the hamsters was directly correlated with the number of cells injected. This new medulloblastoma cell line may prove to be a useful model for experimental brain tumor studies.
从猴腺病毒SA7诱导的仓鼠脑肿瘤中成功建立了髓母细胞瘤细胞的体外培养体系。这些肿瘤细胞在培养物中的形态特征与原始肿瘤非常相似。管状和玫瑰花结生长模式都很明显。在超微结构水平上，肿瘤细胞中仅能检测到少量细胞质细胞器，这是一般未成熟和/或未分化细胞的典型特征。这些肿瘤细胞的增殖依赖于适当密集的初始接种。此外，发现如果用条件培养基处理10天，单个髓母细胞瘤细胞可被刺激产生集落。将这些细胞接种到同基因动物中导致100%摄取。仓鼠的存活与注射的细胞数量直接相关。这种新的髓母细胞瘤细胞系可能被证明是实验性脑肿瘤研究的有用模型。
