Lindberg S, Olsson H
Department of Oto-Rhino-Laryngology, University Hospital, Lund, Sweden.
Acta Otolaryngol. 1991;111(6):1126-32. doi: 10.3109/00016489109100767.
The in vivo effect of tissue kallikrein on the mucociliary activity in the rabbit maxillary sinus was investigated administering the substance (0.1-5 mU/kg) via a. maxillaris and recording the response with a non-invasive photoelectric technique. Tissue kallikrein accelerated mucociliary activity, with a maximum response for the dose 5 mU/kg (33.7 +/- 13.4% from basal levels, n = 5). The effect had a latency of abut 1 min, with a peak within 2-3 min after the beginning of the administration. The response to tissue kallikrein displayed tachyphylaxis with a second dose producing a weaker response. Pretreatment with the protease inhibitor aprotinin (10,000 KIU bolus/kg) inhibited the action of tissue kallikrein. Tissue kallikrein probably stimulates mucociliary activity by producing lysylbradykinin from kininogen. Bradykinin has in an earlier study been shown to stimulate mucociliary activity.
通过上颌动脉给予家兔组织激肽释放酶（0.1 - 5 mU/kg），并用非侵入性光电技术记录反应，以此研究组织激肽释放酶对兔上颌窦黏膜纤毛活动的体内效应。组织激肽释放酶可加速黏膜纤毛活动，5 mU/kg剂量时反应最大（较基础水平增加33.7 +/- 13.4%，n = 5）。该效应有大约1分钟的潜伏期，给药开始后2 - 3分钟内达到峰值。对组织激肽释放酶的反应呈现快速耐受性，第二次给药产生的反应较弱。用蛋白酶抑制剂抑肽酶（10,000 KIU大剂量/kg）预处理可抑制组织激肽释放酶的作用。组织激肽释放酶可能通过从激肽原产生赖氨酰缓激肽来刺激黏膜纤毛活动。在早期研究中已表明缓激肽可刺激黏膜纤毛活动。
