糖尿病与大鼠小肠中葡萄糖转运蛋白基因的表达
Diabetes and glucose transporter gene expression in rat small intestine.
作者信息
Miyamoto K, Hase K, Taketani Y, Minami H, Oka T, Nakabou Y, Hagihira H
机构信息
Department of Nutrition, School of Medicine, University of Tokushima, Japan.
出版信息
Biochem Biophys Res Commun. 1991 Dec 31;181(3):1110-7. doi: 10.1016/0006-291x(91)92053-m.
The expressions of Na(+)-dependent glucose transporter (SGLT1) and five facilitative glucose transporter genes (GLUT1-5) in the small intestine of streptozotocin (STZ)-induced diabetic rats were examined by RNA blotting analysis. The transcripts of SGLT1 mRNA gave bands of 4.5 Kilobases (Kb) and 2.8Kb (very faint band). The levels of SGLT1 mRNA were significantly increased in 30- and 60-day STZ rats, but not changed in acute diabetic rats (2- to 10- day STZ rats). The GLUT2 mRNA levels changed in parallel with the D-galactose transport activity, being increased about 4-fold in 5-day STZ rats. The transcripts of GLUT5 mRNA gave three bands of 5.1Kb, 2.8Kb and 2.OKb, whose levels were significantly reduced in 30- and 60-day STZ rats. These results suggest that the facilitative glucose transporter (GLUT2), in addition to the Na(+)-dependent glucose transporter (SGLT1), may play an important role in intestinal glucose transport in diabetic rats.
通过RNA印迹分析检测了链脲佐菌素（STZ）诱导的糖尿病大鼠小肠中钠依赖性葡萄糖转运体（SGLT1）和五个易化性葡萄糖转运体基因（GLUT1 - 5）的表达。SGLT1 mRNA的转录本产生了4.5千碱基（Kb）和2.8Kb（非常微弱的条带）的条带。SGLT1 mRNA水平在30天和60天的STZ大鼠中显著升高，但在急性糖尿病大鼠（2至10天的STZ大鼠）中未发生变化。GLUT2 mRNA水平与D - 半乳糖转运活性平行变化，在5天的STZ大鼠中增加了约4倍。GLUT5 mRNA的转录本产生了5.1Kb、2.8Kb和2.0Kb的三条带，其水平在30天和60天的STZ大鼠中显著降低。这些结果表明，除了钠依赖性葡萄糖转运体（SGLT1）外，易化性葡萄糖转运体（GLUT2）可能在糖尿病大鼠的肠道葡萄糖转运中起重要作用。
Zotero 插件