Ontawong Atcharaporn, Duangjai Acharaporn, Srimaroeng Chutima
Division of Physiology, School of Medical Sciences, University of Phayao, Muang Phayao, Phayao 56000, Thailand.
Department of Physiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Nong Khai 52000, Thailand.
Biomed Rep. 2021 Sep;15(3):73. doi: 10.3892/br.2021.1449. Epub 2021 Jul 12.
The major constituents of (coffee), including caffeine, chlorogenic acid and caffeic acid, exhibit antihyperglycemic properties in and models. However, whether bean extract (CBE) regulates glucose uptake activity and the underlying mechanisms involved remain unclear. The aim of the present study was to examine the effects of CBE on glucose absorption and identify the mechanisms involved using an model. The uptake of a fluorescent glucose analog into Caco-2 colorectal adenocarcinoma cells was determined. The expression levels of sodium glucose co-transporter 1 (SGLT1) and glucose transporter 2 (GLUT2) were evaluated. In addition, glycoside hydrolase enzyme activity was investigated. It was observed that CBE inhibited disaccharidase enzyme activity. Furthermore, CBE exerted an inhibitory effect on intestinal glucose absorption by downregulating SGLT1- and GLUT2-mediated 5' AMP-activated protein kinase phosphorylation and suppressing hepatocyte nuclear factor 1α expression. These data suggest that CBE may attenuate glucose absorption and may have potentially beneficial antihyperglycemic effects in the body; however, the mechanisms underlying the effects of CBE must be elucidated through further investigation.
咖啡的主要成分，包括咖啡因、绿原酸和咖啡酸，在糖尿病和高血糖模型中表现出降血糖特性。然而，咖啡豆提取物（CBE）是否调节葡萄糖摄取活性以及其中涉及的潜在机制仍不清楚。本研究的目的是使用细胞模型研究CBE对葡萄糖吸收的影响并确定其中涉及的机制。测定了荧光葡萄糖类似物进入Caco-2结肠腺癌细胞的摄取情况。评估了钠葡萄糖共转运蛋白1（SGLT1）和葡萄糖转运蛋白2（GLUT2）的表达水平。此外，还研究了糖苷水解酶活性。观察到CBE抑制双糖酶活性。此外，CBE通过下调SGLT1和GLUT2介导的5'AMP激活蛋白激酶磷酸化并抑制肝细胞核因子1α表达，对肠道葡萄糖吸收产生抑制作用。这些数据表明，CBE可能减弱葡萄糖吸收，并可能在体内具有潜在有益的降血糖作用；然而，CBE作用的潜在机制必须通过进一步研究来阐明。
