Miceli C, La Terza A, Bradshaw R A, Luporini P
Department of Molecular, Cellular and Animal Biology, University of Camerino, Italy.
Eur J Biochem. 1991 Dec 18;202(3):759-64. doi: 10.1111/j.1432-1033.1991.tb16430.x.
The precursors of Euplotes raikovi pheromones Er-2 and Er-10 have been structurally characterized from the sequences of their coding regions that were amplified and cloned using the polymerase chain reaction and oligonucleotide primers corresponding to conserved sequences of the gene for pheromone Er-1. The predicted amino acid sequences contain 75 residues distributed through three domains: signal peptide, pro segment and mature pheromone. Despite the conservation of the overall length, there is variation in the size of the pro segments and of the mature pheromones. The comparison of the sequences shows a gradient of identity from the amino to the carboxyl terminus; the signal sequences are identical (with greater than or equal to 95% identity in the nucleotide sequences), the pro segments more variable and the mature pheromones quite diverse. The processing site of the pro pheromones, to produce the mature forms, is apparently characterized by the unusual Xaa-Asp sequence.
利用聚合酶链反应以及与信息素Er-1基因保守序列对应的寡核苷酸引物，通过扩增和克隆，从编码区序列中对雷氏游仆虫信息素Er-2和Er-10的前体进行了结构表征。预测的氨基酸序列包含75个残基，分布在三个结构域：信号肽、前体片段和成熟信息素。尽管总长度保守，但前体片段和成熟信息素的大小存在差异。序列比较显示从氨基端到羧基端存在同一性梯度；信号序列相同（核苷酸序列同一性大于或等于95%），前体片段更具变异性，成熟信息素则差异很大。前体信息素产生成熟形式的加工位点显然以不寻常的Xaa-Asp序列为特征。
