Miceli C, La Terza A, Melli M
Department of Cell Biology, University of Camerino, Italy.
Proc Natl Acad Sci U S A. 1989 May;86(9):3016-20. doi: 10.1073/pnas.86.9.3016.
cDNA clones comprising the entire coding region for the mating pheromone Er-1 of Euplotes raikovi have been isolated by oligonucleotide screening of two cDNA libraries in the vectors lambda gt10 and pUC12. The cDNA sequence contains an open reading frame of 75 amino acids that constitute pre-pro-Er-1. The amino acid sequence of secreted Er-1 starts at aspartic acid-36 of pre-pro-Er-1 and completely matches that known by direct Er-1 protein sequencing. The coding region of Er-1 cDNA ends with codon TAA, which specifies glutamine in other ciliates. The 5'- and 3'-noncoding regions contain, respectively, two and one inverted repeats. The 3'-noncoding-region inverted repeat, which includes the unusual polyadenylylation signal AACAAA, has been related to RNA 3'-terminus formation.
通过对λgt10和pUC12载体中的两个cDNA文库进行寡核苷酸筛选，已分离出包含莱氏游仆虫交配信息素Er-1完整编码区的cDNA克隆。该cDNA序列包含一个由75个氨基酸组成的开放阅读框，构成前体-前-Er-1。分泌型Er-1的氨基酸序列从前体-前-Er-1的天冬氨酸-36开始，与通过直接对Er-1蛋白测序所知的序列完全匹配。Er-1 cDNA的编码区以密码子TAA结束，该密码子在其他纤毛虫中编码谷氨酰胺。5'-和3'-非编码区分别包含两个和一个反向重复序列。3'-非编码区反向重复序列，包括不寻常的聚腺苷酸化信号AACAAA，与RNA 3'-末端形成有关。
