Wang Mingming, Zhang Daliang, Zhang Hong ping, Wang Zhe, Chen Xuemei, Jian Jing fen
Department of Otolaryngology, the Second Hospital of Shandong University, Jinan, 250033, China.
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2006 Oct;20(20):914-6.
To study the role of superantigens produced by Staphylococcus aureus in the pathogenesis of nasal polyps.
sinonasal mucus and polyp/mucosa tissue were obtained from sixty-nine patients with chronic rhinosinusitis (42 patients with bilateral nasal polyps, 27 without nasal polyps). Samples from twelve normal subjects were also obtained for comparative negative controls. Mucus specimens were cultured for bacteria. Tissue specimens were assayed by ELISA for staphylococcal exotoxins. In addition, the histopathology of nasal polyps was studied. The paraffin sections were stained with hematoxylin and eosin, and these were examined for eosinophil counts.
The percentages of Staphylococcus aureus in sinonasal mucus were 7.1 for chronic rhinosinusitis with nasal polyp (CRSwNP) subjects, 3.7% for chronic rhinosinusitis without nasal polyps (CRSsNP). and 0 for controls respectively. There were no significant differences between these groups. In CRSwNP subjects 23 of 42 samples (54.76%) demonstrated reactivity for at least one staphylococcal exotoxin. There were no positive results in the CRSsNP or control groups. The mean eosinophil counts were 23. 94+/-13. 88 in CRSwNP group, 0.29+/-0.51 in CRSsNP group, and 0. 08+/-0.28 in controls. The differences in these three groups were all significant ( P <0.05). There was a trend toward higher eosinophil levels in polyps of ELISA-positive patients compared with that of ELISA-negative patients, although this was not statistically different ( P >0.05).
There were correlation between Staphylococcal superantigens and nasal CRSwNP, and superantigens were possible etiological agents in the pathogenesis of CRSwNP.
研究金黄色葡萄球菌产生的超抗原在鼻息肉发病机制中的作用。
从69例慢性鼻窦炎患者（42例双侧鼻息肉患者，27例无鼻息肉患者）获取鼻窦黏液及息肉/黏膜组织。还获取了12例正常受试者的样本作为比较阴性对照。黏液标本进行细菌培养。组织标本通过酶联免疫吸附测定法检测葡萄球菌外毒素。此外，对鼻息肉进行组织病理学研究。石蜡切片用苏木精和伊红染色，并检查嗜酸性粒细胞计数。
鼻息肉慢性鼻窦炎（CRSwNP）患者鼻窦黏液中金黄色葡萄球菌的比例为7.1%，无鼻息肉慢性鼻窦炎（CRSsNP）患者为3.7%，对照组为0。这些组之间无显著差异。在CRSwNP患者中，42个样本中有23个（54.76%）对至少一种葡萄球菌外毒素呈反应性。CRSsNP组或对照组无阳性结果。CRSwNP组嗜酸性粒细胞平均计数为23.94±13.88，CRSsNP组为0.29±0.51，对照组为0.08±0.28。这三组之间的差异均有统计学意义（P<0.05）。ELISA阳性患者息肉中的嗜酸性粒细胞水平有高于ELISA阴性患者的趋势，尽管无统计学差异（P>0.05）。
葡萄球菌超抗原与鼻CRSwNP之间存在相关性，超抗原可能是CRSwNP发病机制中的病因。
