Batter D K, Kessler J A
Department of Neurology, Albert Einstein College of Medicine, Bronx, NY 10461.
Brain Res Mol Brain Res. 1991 Aug;11(1):65-9. doi: 10.1016/0169-328x(91)90022-p.
Regulation of preproenkephalin (PPE) mRNA was examined in astrocytes cultured from several regions of the neonatal rat brain. Astrocytes from these regions expressed differing levels of PPE mRNA, with higher levels in astrocytes from the hypothalamus followed by frontal cortex and striatum. Further, PPE mRNA was regulated differently in hypothalamic than in striatal glia. Treatment of striatal astrocytes with the beta-adrenergic agonist, isoproterenol, or with agents which directly increased intracellular cAMP (forskolin or 8-bromo-cAMP) elevated levels of PPE mRNA. By contrast, none of these treatments altered levels of PPE mRNA in hypothalamic astrocytes despite increasing cAMP levels 60-fold. These observations indicate that there is striking regional heterogeneity in the expression and regulation of PPE mRNA by astrocytes, suggesting that proenkephalin or its derived peptides help to mediate region-specific brain functions.
研究了新生大鼠脑不同区域培养的星形胶质细胞中前脑啡肽原（PPE）mRNA的调控情况。这些区域的星形胶质细胞表达不同水平的PPE mRNA，下丘脑星形胶质细胞中的水平较高，其次是额叶皮质和纹状体。此外，下丘脑和纹状体神经胶质细胞中PPE mRNA的调控方式不同。用β-肾上腺素能激动剂异丙肾上腺素或直接增加细胞内cAMP的试剂（福斯高林或8-溴-cAMP）处理纹状体星形胶质细胞，可提高PPE mRNA水平。相比之下，尽管cAMP水平增加了60倍，但这些处理均未改变下丘脑星形胶质细胞中PPE mRNA的水平。这些观察结果表明，星形胶质细胞对PPE mRNA的表达和调控存在显著的区域异质性，提示脑啡肽原或其衍生肽有助于介导区域特异性脑功能。
