[反相高效液相色谱法同时测定牡丹皮中三种活性成分]
[Simultaneous determination of three active constituents in cortex moutan by RP-HPLC].
作者信息
Wang Yu-Wen, Yu Ke, Cheng Yi-Yu
机构信息
Department of Chinese Medicine Science and Engineering, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310027, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2006 Oct;31(20):1699-701.
OBJECTIVE
To establish a RP-HPLC method for the simultaneous determination of gallic acid, paeoniflorin and paeonol in Cortex Moutan.
METHOD
The analysis was carried out on a column of YMC-C18 (4. 6 mm x 250 mm, 5 microm), and gradient elution was used for the baseline separation of these three constituents with the different detection wavelength.
RESULT
The linear ranges for gallic acid, paeoniflorin and paeonol were 0.0130-0.267, 0.0880-1.76 and 0.164-3.28 microg, respectively. Their average recoveries were 104.3%, 101.7% and 99.5%, respectively.
CONCLUSION
The method was successfully applied to the determination of gallic acid, paeoniflorin and paeonol in Cortex Moutan from the different drugstores. It was shown in this study that the proposed method could be used to evaluate the quality of the medicinal plants or herbal preparations containing gallic acid, paeoniflorin and paeonol.
目的
建立同时测定牡丹皮中没食子酸、芍药苷和丹皮酚含量的反相高效液相色谱法。
方法
采用YMC-C18柱（4.6 mm×250 mm，5μm），梯度洗脱，在不同检测波长下对这3种成分进行基线分离。
结果
没食子酸、芍药苷和丹皮酚的线性范围分别为0.0130 - 0.267μg、0.0880 - 1.76μg和0.164 - 3.28μg，平均回收率分别为104.3%、101.7%和99.5%。
结论
该方法成功应用于不同药店牡丹皮中没食子酸、芍药苷和丹皮酚的含量测定。本研究表明，该方法可用于评价含有没食子酸、芍药苷和丹皮酚的药用植物或草药制剂的质量。
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