一种经过验证的液相色谱法，用于测定各种茄属植物及市售制剂中的澳洲茄碱。

A validated liquid chromatographic method for estimation of solasodine in various Solanum species and market formulations.

作者信息

Trivedi Priti D, Patel Brijesh C, Rathnam Shivprakash, Pundarikakshudu Kilambi

机构信息

K.B. Institute of Pharmaceutical Education and Research, Sector-23, GH-6, Gandhinagar PIN Code 382023, Gujarat, India.

出版信息

J AOAC Int. 2006 Nov-Dec;89(6):1519-23.

PMID:17225596

Abstract

This paper describes an improved liquid chromatographic (LC) method involving a prechromatographic derivatization step for the estimation of solasodine from berries of various Solanum species, market samples of Solanum xanthocarpum herb, extract, and its market formulations. Solasodine has heterocyclic nitrogen but has no conjugated double bonds in its structure. However, in all reported LC methods, detection was made in the ultraviolet range of 200-213 nm. In the present study, a prechromatographic derivatization of solasodine was done by forming an ion-pair complex of the heterocyclic nitrogen using the acidic dye methyl orange and acetate buffer of pH 4.7. Detection could be made in the visible range at 530 nm in this method. The method was validated and successfully applied to determine solasodine content in various plant samples and polyherbal formulations. The relative standard deviation was found to be 0.025% for system precision, and 0.8% for the linearity of the method, and the correlation coefficient was 0.999. Plant samples and market formulations were analyzed and found to contain solasodine in the range of 0.113-0.227% (w/w) on a fresh weight basis in berries; 0.3-1.278% (w/w) and 0.412% (w/w) on a dry weight basis in S. xanthocarpum herb powder and extract, respectively; and 0.245-0.525% (w/w) on dry weight basis in formulations containing S. xanthocarpum herb powder. No matrix interference was encountered. The method was found to be accurate, with a mean recovery of 100.5 +/- 0.83%. The method has good reproducibility and was found to be suitable for estimation of solasodine.

摘要

本文描述了一种改进的液相色谱（LC）方法，该方法涉及一个色谱前衍生步骤，用于测定各种茄属植物果实、黄果茄草药的市售样品、提取物及其市售制剂中的茄解碱。茄解碱含有杂环氮，但结构中没有共轭双键。然而，在所有已报道的LC方法中，检测都是在200 - 213 nm的紫外范围内进行的。在本研究中，通过使用酸性染料甲基橙和pH 4.7的醋酸盐缓冲液形成杂环氮的离子对络合物，对茄解碱进行色谱前衍生。该方法可在530 nm的可见光范围内进行检测。该方法经过验证，并成功应用于测定各种植物样品和多草药制剂中的茄解碱含量。系统精密度的相对标准偏差为0.025%，方法线性的相对标准偏差为0.8%，相关系数为0.999。对植物样品和市售制剂进行分析，发现浆果中以鲜重计茄解碱含量在0.113 - 0.227%（w/w）范围内；黄果茄草药粉末和提取物中以干重计分别为0.3 - 1.278%（w/w）和0.412%（w/w）；含有黄果茄草药粉末的制剂中以干重计为0.245 - 0.525%（w/w）。未遇到基质干扰。该方法被发现是准确的，平均回收率为100.5±0.83%。该方法具有良好的重现性，适用于茄解碱的测定。