Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Talanta. 2010 Apr 15;81(1-2):565-71. doi: 10.1016/j.talanta.2009.12.044. Epub 2010 Jan 4.
The development of sequential injection analysis with lab-at-valve (LAV) semi-automated system on-line liquid-liquid extraction is demonstrated for spectrophotometric determination of solasodine in various Solanum species fruits. The main proposed is semi-automated extractive determination of solasodine using methyl orange as colorimetric reagent. After optimization of the system, sample, reagent and organic solvent were sequentially aspirated into an extraction coil connected to the center of a selection valve, where extraction took place by flow reversal. The aqueous and organic phases were separated in a lab-at-valve unit attracted to one of the ports of the selection valve. The absorption of ion-pair solasodine-methyl orange complex in the organic phase was measured spectrophotometrically at 420 nm. The method performances, including reproducibility, linearity, sensitivity and accuracy, were also evaluated. The proposed method is simple, reproducible and accurate. It was successfully applied to the determination of solasodine in Solanum aculeatissimum Jacq., Solanum violaceum Ortega., Solanum melongena Linn. and Solanum indicum Linn. fruits in Solanaceae family. Results obtained were in good agreement with those obtained by batch wise spectrophotometric method. It is also suitable and useful for determination of solasodine in other medicinal plants.
本文建立了阀上实验室（LAV）半自动顺序注射在线液-液萃取分光光度法用于不同茄属植物果实中甾体皂甙元-茄碱的测定。主要采用甲基橙为显色剂，提出了半自动萃取测定方法。在系统优化后，样品、试剂和有机溶剂被顺序吸入与选择阀中心相连的萃取盘管中，通过流路切换进行萃取。在阀上实验室单元中，水相和有机相在选择阀的一个端口处被分离。在 420nm 波长处测定离子对缔合物-甲基橙在有机相中的吸收。对方法的性能，包括重现性、线性、灵敏度和准确度进行了评估。该方法简单、重现性好、准确度高。成功地应用于测定茄科植物刺天茄、少花龙葵、茄子和野茄果实中的茄碱。所得结果与分批分光光度法一致。该方法也适用于其他药用植物中茄碱的测定。
