Rudel D, Bathori M, Gharbi J, Girault J P, Racz I, Melis K, Szendrei K, Lafont R
Ecole Normale Supérieure, Département de Biologie, Laboratoire de Biochimie et Physiologie du Développement, CNRS URA 686, 46 rue d'Ulm, F-75230 Paris Cedex 05, France.
Planta Med. 1992 Aug;58(4):358-64. doi: 10.1055/s-2006-961485.
Six new ecdysteroids have been isolated from SERRATULA TINCTORIA; these are: the 2,22- and 3,22-diacetates of 20-hydroxyecdysone, 5beta-hydroxyrubrosterone, 3-epi-poststerone, 3-epi-rubrosterone, and 22-oxo-20-hydroxyecdysone. These minor compounds were found together with the known ecdysteroids, 20-hydroxyecdysone, its 2-, 3-, and 22-monoacetates, rubrosterone, poststerone, polypodine B (5beta,20-dihydroxyecdysone), pterosterone (25-deoxy-20,24-dihydroxyecdysone), and makisterone C (24-ethyl-20-hydroxyecdysone). All these ecdysteroids were isolated by a combination of several chromatographic techniques (liquid chromatography on alumina, DCCC, and HPLC), then identified using standard mass spectrometric and 2D (1)H-NMR techniques.
从染用麻花头中分离出了六种新的蜕皮甾体；它们分别是：20-羟基蜕皮酮的2,22-二乙酸酯和3,22-二乙酸酯、5β-羟基红甾酮、3-表-后甾酮、3-表-红甾酮以及22-氧代-20-羟基蜕皮酮。这些次要化合物是与已知的蜕皮甾体一同被发现的，已知的蜕皮甾体有：20-羟基蜕皮酮、其2-、3-和22-单乙酸酯、红甾酮、后甾酮、多足甾酮B（5β,20-二羟基蜕皮酮）、翼甾酮（25-脱氧-20,24-二羟基蜕皮酮）以及玛氏甾酮C（24-乙基-20-羟基蜕皮酮）。所有这些蜕皮甾体都是通过多种色谱技术（氧化铝柱液相色谱、DCCC和HPLC）相结合的方法分离得到的，然后使用标准质谱和二维(1)H-NMR技术进行鉴定。
