Department of Organic Chemistry, Royal Danish School of Pharmacy, Universitelsparken 2, DK-2100 Copenhagen, Denmark.
Planta Med. 1992 Oct;58(5):454-8. doi: 10.1055/s-2006-961512.
Plants belonging to Malvaceae (about 90 species belonging to 33 genera) were analysed for the presence of gossypol by reversed-phase HPLC using absorbance detection at 365 nm, and the chirality and optical purity of gossypol were determined whenever the analysis was positive. Only plants belonging to the cotton tribe (Gossypieae) contained gossypol (detection limit better than 0.001%). In particular, no gossypol could be detected in ABELMOSCHUS ESCULENTUS (ocra), HIBISCUS TILIACEUS, H. SABDARIFFA, and HEREA BRASI-LIENSIS, earlier claimed to contain this compound. Gossypol-containing plants usually produced dextrorotatory gossypol of varying optical purity; an enantiomeric excess of (-)-gossypol was detected in only one plant, GOSSYPIUTN BARBADENSE.
用反相高效液相色谱法，在 365nm 处用吸光度检测，对属于锦葵科（约 33 属 90 种）的植物进行了棉酚的存在分析，只要分析结果为阳性，就确定棉酚的手性和光学纯度。只有属于棉花族（棉属）的植物含有棉酚（检测限优于 0.001%）。特别是，在 ABELMOSCHUS ESCULENTUS（ocra）、HIBISCUS TILIACEUS、H. SABDARIFFA 和 HEREA BRASI-LIENSIS 中，均未检测到以前声称含有这种化合物的棉酚。通常，含有棉酚的植物会产生不同光学纯度的右旋棉酚；仅在一种植物 GOSSYPIUTN BARBADENSE 中检测到（-）-棉酚的对映体过量。
