Chancerelle Y, Alban C, Viret R, Tosetti F, Kergonou J F
Centre de Recherches du Service de Santé des Armées, La Tronche, France.
Biochem Int. 1991 May;24(1):157-63.
Reactions of MDA with primary amino groups produce inter- or intra-molecular 1-amino-3-imino-propene (AIP) bridges, leading to structural modifications of biological molecules. In this work, applying electrophoresis followed by transfer onto nitrocellulose membranes, we observed that serum of a rabbit immunized with MDA-modified lysozyme (ML) reacts not only with ML and native lysozyme (L), but also with MDA-modified ribonuclease, cytochrome c or polylysine (MR, MC and MP respectively), while it does not react with native ribonuclease, cytochrome c or polylysine (R, C and P respectively). These results confirm previous ones indicating that sera of rabbits immunized with ML contain antibodies reacting specifically with epitopes containing AIP bridges.
丙二醛（MDA）与伯氨基的反应会产生分子间或分子内的1-氨基-3-亚氨基丙烯（AIP）桥，从而导致生物分子的结构发生改变。在本研究中，我们采用电泳后转印至硝酸纤维素膜的方法，观察到用MDA修饰的溶菌酶（ML）免疫的兔血清不仅能与ML和天然溶菌酶（L）发生反应，还能与MDA修饰的核糖核酸酶、细胞色素c或聚赖氨酸（分别为MR、MC和MP）发生反应，而不与天然核糖核酸酶、细胞色素c或聚赖氨酸（分别为R、C和P）发生反应。这些结果证实了之前的研究结果，即用ML免疫的兔血清中含有能与含AIP桥的表位特异性反应的抗体。
