Kergonou J F, Pennacino I, Lafite C, Ducousso R
Centre de Recherches du Service de Santé des Armées, Clamart, France.
Biochem Int. 1988 May;16(5):845-52.
Using immunoenzymatic reactions (ELISA) with MDA-crosslinked lysozyme (ML) or poly-L-lysine (MP) and with the corresponding native protein or polypeptide, we showed that sera from normal human subjects contain immunoglobulins with antibody-like specificity for MDA-modified proteins. Inhibition studies with ML and MP showed that the chemical structures recognized by these immunoglobulins include 1-amino-3-iminopropene (AIP) bridges resulting from reactions of MDA with primary amino groups. An ELISA technique for quantitation of these immunoglobulins was developed and applied to 32 sera from healthy humans, which provided an estimation of their normal levels. Variations of these levels under pathological conditions are now being investigated.
利用免疫酶反应（ELISA），分别使用丙二醛交联溶菌酶（ML）或聚-L-赖氨酸（MP）以及相应的天然蛋白质或多肽，我们发现正常人类受试者的血清中含有对丙二醛修饰蛋白具有抗体样特异性的免疫球蛋白。用ML和MP进行的抑制研究表明，这些免疫球蛋白识别的化学结构包括丙二醛与伯氨基反应产生的1-氨基-3-亚氨基丙烯（AIP）桥。我们开发了一种用于定量这些免疫球蛋白的ELISA技术，并将其应用于32名健康人的血清，从而估算出它们的正常水平。目前正在研究这些水平在病理条件下的变化。
