Herosimczyk A, Dejeans N, Sayd T, Ozgo M, Skrzypczak W F, Mazur A
Department of Animal Physiology and Cytobiology, Agricultural University, Szczecin, Poland.
J Physiol Pharmacol. 2006 Nov;57 Suppl 7:81-93.
The knowledge of concentration, modification and interaction of proteins is fundamental in determining the phenotype of living organisms. Plasma, the primary clinical specimen, contains numerous and diverse proteins. The functions of these proteins are as manifold as the diversity of the protein themselves. Many of them have been largely used for many years as biomarkers of diseases and indicators of the physiological functions. The study of plasma proteome promises to be a significant advance in various areas of biological and clinical research. Two-dimensional polyacrylamide gel electrophoresis is considered as a primary tool in separating thousand of plasma proteins. This approach enables comparing normal and diseased samples revealing differently expressed proteins. Other proteomic techniques suitable for plasma analysis such as protein microarrays are now either established or are still being improved. This article briefly reviews the application of two-dimensional electrophoresis and the current status of technical aspects for plasma proteome.
蛋白质的浓度、修饰和相互作用的知识是确定生物体表型的基础。血浆作为主要的临床样本，含有大量多样的蛋白质。这些蛋白质的功能与蛋白质本身的多样性一样繁多。多年来，其中许多蛋白质已被广泛用作疾病的生物标志物和生理功能的指标。血浆蛋白质组学的研究有望在生物和临床研究的各个领域取得重大进展。二维聚丙烯酰胺凝胶电泳被认为是分离数千种血浆蛋白的主要工具。这种方法能够比较正常样本和患病样本，揭示差异表达的蛋白质。其他适用于血浆分析的蛋白质组学技术，如蛋白质微阵列，目前要么已经建立，要么仍在改进。本文简要回顾了二维电泳的应用以及血浆蛋白质组学技术方面的现状。
