Thadikkaran Lynne, Siegenthaler Michèle A, Crettaz David, Queloz Pierre-Alain, Schneider Philippe, Tissot Jean-Daniel
Service régional vaudois de transfusion sanguine, Rue du Bugnon 27, CH-1005 Lausanne, Switzerland.
Proteomics. 2005 Aug;5(12):3019-34. doi: 10.1002/pmic.200402053.
Blood is divided in two compartments, namely, plasma and cells. The latter contain red blood cells, leukocytes, and platelets. From a descriptive medical discipline, hematology has evolved towards a pioneering discipline where molecular biology has permitted the development of prognostic and diagnostic indicators for disease. The recent advance in MS and protein separation now allows similar progress in the analysis of proteins. Proteomics offers great promise for the study of proteins in plasma/serum, indeed a number of proteomics databases for plasma/serum have been established. This is a very complex body fluid containing lipids, carbohydrates, amino acids, vitamins, nucleic acids, hormones, and proteins. About 1500 different proteins have recently been identified, and a number of potential new markers of diseases have been characterized. Here, examples of the enormous promise of plasma/serum proteomic analysis for diagnostic/prognostic markers and information on disease mechanism are given. Within the blood are also a large number of different blood cell types that potentially hold similar information. Proteomics of red blood cells, until now, has not improved our knowledge of these cells, in contrast to the major progresses achieved while studying platelets and leukocytes. In the future, proteomics will change several aspects of hematology.
血液分为两个部分，即血浆和细胞。后者包含红细胞、白细胞和血小板。血液学已从一门描述性医学学科发展成为一门前沿学科，分子生物学使疾病的预后和诊断指标得以发展。质谱和蛋白质分离技术的最新进展现在使蛋白质分析取得了类似的进展。蛋白质组学为血浆/血清中蛋白质的研究带来了巨大希望，实际上已经建立了许多血浆/血清蛋白质组学数据库。这是一种非常复杂的体液，含有脂质、碳水化合物、氨基酸、维生素、核酸、激素和蛋白质。最近已鉴定出约1500种不同的蛋白质，并且已经鉴定出许多潜在的疾病新标志物。这里给出了血浆/血清蛋白质组学分析在诊断/预后标志物以及疾病机制信息方面具有巨大潜力的实例。血液中还存在大量不同类型的血细胞，它们可能包含类似的信息。与研究血小板和白细胞时取得的重大进展相比，到目前为止，红细胞蛋白质组学尚未增进我们对这些细胞的了解。未来，蛋白质组学将改变血液学的多个方面。
