[采用高效液相色谱法测定全血中的组胺和5-羟色胺]
[Determination of histamine and serotonin in whole blood using the method of high performance liquid chromatography].
作者信息
Akmedzhanova S B, Balabolkin I I, Vasil'eva E M, Eller K I
出版信息
Lab Delo. 1991(11):10-3.
The authors have developed a method for measuring histamine and serotonin in whole blood, based on reverse-phase high-performance liquid chromatography of 5-dimethylaminonaphthyl sulfonic (DNS) amine derivatives with fluorometric detection. Chromatography in a mini-column packed with polyamide was employed for the isolation and purification of DNS-histamine and serotonin. The lowest detectable level of histamine is 2-2.5 ng/ml of blood, that of serotonin 1-1.5 ng/ml. The method was tried with whole blood samples from children suffering from various allergic conditions. Whole blood histamine and serotonin concentration were found particularly elevated in the patients with gastrointestinal allergies.
作者基于用荧光检测的5-二甲基氨基萘基磺酸（DNS）胺衍生物的反相高效液相色谱法，开发了一种测量全血中组胺和血清素的方法。使用填充有聚酰胺的微型柱进行色谱分离，以分离和纯化DNS-组胺和血清素。组胺的最低可检测水平为每毫升血液2 - 2.5纳克，血清素为每毫升血液1 - 1.5纳克。该方法已用于患有各种过敏病症儿童的全血样本检测。发现患有胃肠道过敏的患者全血组胺和血清素浓度特别升高。
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