McMillan James R, Akiyama Masashi, Tanaka Masaru, Yamamoto Sadaki, Goto Maki, Abe Riichiro, Sawamura Daisuke, Shimomura Masatsugu, Shimizu Hiroshi
Department of Dermatology, Hokkaido University, Graduate School of Medicine, Sapporo, Japan.
Tissue Eng. 2007 Apr;13(4):789-98. doi: 10.1089/ten.2006.0321.
Autologous keratinocyte grafts provide clinical benefit by rapidly covering wounded areas, but they are fragile. We therefore developed biocompatible hexagonal-packed porous films with uniform, circular pore sizes to support human keratinocytes and fibroblasts. Cells were cultured on these porous poly (epsilon-calprolactone) films with pore sizes ranging from novel ultra-small 3 microm to 20 microm. These were compared with flat (pore-less) films. Cell growth rates, adhesion, migration, and ultrastructural morphology were examined. Human keratinocytes and fibroblasts attached to all films. Furthermore, small-pore (3-5 microm) films showed the highest levels of cell adhesion and survival and prevented migration into the pores and opposing film surface. Keratinocyte migration over small-pore film surface was inhibited. Keratinocytes optimally attached to 3-microm-pore films due to a combination of greater pore numbers (porosity), a greater circumference of the pore edge per unit surface area, and greater frequency of flat surface areas for attachment, allowing better cell-substrate and cell-cell attachment and growth. The 3-microm pore size allowed cell-cell communication, together with diffusion of soluble nutrients and factors from the culture medium or wound substrate. These characteristics are considered important in developing grafts for use in the treatment of human skin wounds.
自体角质形成细胞移植物通过快速覆盖伤口区域提供临床益处，但它们很脆弱。因此，我们开发了具有均匀圆形孔径的生物相容性六方堆积多孔薄膜，以支持人角质形成细胞和成纤维细胞。将细胞培养在这些孔径范围从新型超小的3微米到20微米的多孔聚（ε-己内酯）薄膜上。将这些与平的（无孔）薄膜进行比较。检测细胞生长速率、粘附、迁移和超微结构形态。人角质形成细胞和成纤维细胞附着于所有薄膜。此外，小孔径（3 - 5微米）薄膜显示出最高水平的细胞粘附和存活，并防止细胞迁移到孔中和相对的薄膜表面。角质形成细胞在小孔径薄膜表面的迁移受到抑制。角质形成细胞最佳地附着于3微米孔径的薄膜，这是由于更大的孔数量（孔隙率）、单位表面积更大的孔边缘周长以及更大频率的平坦附着表面区域的组合，从而允许更好的细胞 - 底物和细胞 - 细胞附着及生长。3微米的孔径允许细胞 - 细胞通讯，以及可溶性营养物质和因子从培养基或伤口底物的扩散。这些特性在开发用于治疗人类皮肤伤口的移植物中被认为是重要的。
