Neumann Gabriele, Geisbert Thomas W, Ebihara Hideki, Geisbert Joan B, Daddario-DiCaprio Kathleen M, Feldmann Heinz, Kawaoka Yoshihiro
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 2015 Linden Drive, Madison, WI 53706, USA.
J Virol. 2007 Mar;81(6):2995-8. doi: 10.1128/JVI.02486-06. Epub 2007 Jan 17.
Enveloped viruses often require cleavage of a surface glycoprotein by a cellular endoprotease such as furin for infectivity and virulence. Previously, we showed that Ebola virus glycoprotein does not require the furin cleavage motif for virus replication in cell culture. Here, we show that there are no appreciable differences in disease progression, hematology, serum biochemistry, virus titers, or lethality in nonhuman primates infected with an Ebola virus lacking the furin recognition sequence compared to those infected with wild-type virus. We conclude that glycoprotein cleavage by subtilisin-like endoproteases is not critical for Ebola virus infectivity and virulence in nonhuman primates.
包膜病毒通常需要细胞内蛋白酶（如弗林蛋白酶）切割表面糖蛋白才能具有感染性和毒力。此前，我们发现埃博拉病毒糖蛋白在细胞培养中进行病毒复制时不需要弗林蛋白酶切割基序。在此，我们表明，与感染野生型病毒的非人灵长类动物相比，感染缺乏弗林蛋白酶识别序列的埃博拉病毒的非人灵长类动物在疾病进展、血液学、血清生物化学、病毒滴度或致死率方面没有明显差异。我们得出结论，枯草杆菌蛋白酶样内蛋白酶切割糖蛋白对埃博拉病毒在非人灵长类动物中的感染性和毒力并不关键。
