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基于重组蛋白的检测严重急性呼吸综合征冠状病毒刺突蛋白和核衣壳蛋白抗体的检测方法。

Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins.

作者信息

Haynes Lia M, Miao Congrong, Harcourt Jennifer L, Montgomery Joel M, Le Mai Quynh, Dryga Sergey A, Kamrud Kurt I, Rivers Bryan, Babcock Gregory J, Oliver Jennifer Betts, Comer James A, Reynolds Mary, Uyeki Timothy M, Bausch Daniel, Ksiazek Thomas, Thomas William, Alterson Harold, Smith Jonathan, Ambrosino Donna M, Anderson Larry J

机构信息

National Centers for Immunization and Respiratory Diseases, Division of Viral Diseases, Respiratory and Gastroenteritis Viruses Branch, Centers for Disease Control and Prevention, 1600 Clifton Rd. NE, Mailstop G-18, Atlanta, GA 30333.

出版信息

Clin Vaccine Immunol. 2007 Mar;14(3):331-3. doi: 10.1128/CVI.00351-06. Epub 2007 Jan 17.

DOI:10.1128/CVI.00351-06
PMID:17229882
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1828864/
Abstract

Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

摘要

研发并评估了基于重组严重急性呼吸综合征（SARS）核衣壳蛋白和刺突蛋白的免疫球蛋白G免疫测定法。我们的测定法对症状出现后长达2年的患者血清中的SARS冠状病毒显示出高灵敏度和特异性。这些测定法不仅将有助于常规SARS冠状病毒诊断，还将有助于流行病学和抗体动力学研究。

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Clin Vaccine Immunol. 2007 Mar;14(3):331-3. doi: 10.1128/CVI.00351-06. Epub 2007 Jan 17.
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本文引用的文献

1
False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid-based western blot assay were rectified by the use of two subunits (S1 and S2) of spike for detection of antibody to SARS-CoV.在基于重组严重急性呼吸综合征相关冠状病毒（SARS-CoV）核衣壳的蛋白质印迹分析中，通过使用刺突蛋白的两个亚基（S1和S2）来检测抗SARS-CoV抗体，纠正了假阳性结果。
Clin Vaccine Immunol. 2006 Mar;13(3):409-14. doi: 10.1128/CVI.13.3.409-414.2006.
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Two-year prospective study of the humoral immune response of patients with severe acute respiratory syndrome.重症急性呼吸综合征患者体液免疫反应的两年前瞻性研究
J Infect Dis. 2006 Mar 15;193(6):792-5. doi: 10.1086/500469. Epub 2006 Feb 9.
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Longitudinally profiling neutralizing antibody response to SARS coronavirus with pseudotypes.使用假型对严重急性呼吸综合征冠状病毒的中和抗体反应进行纵向分析。
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The epitope study on the SARS-CoV nucleocapsid protein.严重急性呼吸综合征冠状病毒核衣壳蛋白的表位研究
Genomics Proteomics Bioinformatics. 2003 Aug;1(3):198-206. doi: 10.1016/s1672-0229(03)01025-8.
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The C-terminal portion of the nucleocapsid protein demonstrates SARS-CoV antigenicity.核衣壳蛋白的C末端部分表现出SARS-CoV抗原性。
Genomics Proteomics Bioinformatics. 2003 Aug;1(3):193-7. doi: 10.1016/s1672-0229(03)01024-6.
6
False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide.在重组严重急性呼吸综合征相关冠状病毒（SARS-CoV）核衣壳酶联免疫吸附测定中，因HCoV-OC43和HCoV-229E导致的假阳性结果，通过用重组SARS-CoV刺突多肽进行蛋白质免疫印迹法得以纠正。
J Clin Microbiol. 2004 Dec;42(12):5885-8. doi: 10.1128/JCM.42.12.5885-5888.2004.
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Laboratory diagnosis of four recent sporadic cases of community-acquired SARS, Guangdong Province, China.中国广东省近期4例社区获得性SARS散发病例的实验室诊断
Emerg Infect Dis. 2004 Oct;10(10):1774-81. doi: 10.3201/eid1010.040445.
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