Garrison C J, Dougherty P M, Kajander K C, Carlton S M
Department of Anatomy and Neuroscience, University of Texas Medical Branch, Galveston 77550.
Brain Res. 1991 Nov 22;565(1):1-7. doi: 10.1016/0006-8993(91)91729-k.
The change in staining density of glial fibrillary acidic protein (GFAP) was analyzed in rats that sustained a chronic constriction injury produced by sutures tied loosely around one sciatic nerve. This injury model of peripheral neuropathy resulted in a behavioral hyperalgesia evidenced by a decrease in mean foot withdrawal latency to radiant heat. Increased GFAP immunostaining was observed in the gray matter of the spinal cord ipsilateral to the lesion and specific to spinal segments in which the sciatic nerve is distributed. Elevated GFAP staining density was attributed primarily to hypertrophy of astrocytes rather than their proliferation or migration since counts of astrocyte profiles demonstrated no significant difference when comparing the lesioned to the control side. The magnitude of the increase in GFAP staining correlated with the degree of hyperalgesia. Thus, these data suggest that astrocytes participate in the sequelae occurring in the dorsal horn following constriction injury of a peripheral nerve.
在大鼠中分析了胶质纤维酸性蛋白（GFAP）染色密度的变化，这些大鼠通过在一条坐骨神经周围松散结扎缝线造成慢性压迫性损伤。这种周围神经病变的损伤模型导致行为性痛觉过敏，表现为对辐射热的平均足退缩潜伏期缩短。在损伤同侧脊髓灰质以及坐骨神经分布的特定脊髓节段中观察到GFAP免疫染色增加。GFAP染色密度升高主要归因于星形胶质细胞肥大，而非其增殖或迁移，因为比较损伤侧和对照侧的星形胶质细胞轮廓计数未显示出显著差异。GFAP染色增加的幅度与痛觉过敏程度相关。因此，这些数据表明星形胶质细胞参与了周围神经压迫性损伤后背角出现的后遗症。
