Tai Wenjiao, Shang Junkui, Zhao Peiqi, Li Wei, Shen Tianjin, Zhong Xiaoling, Zou Yuhua, Chen Bo, Zhang Chun-Li
Department of Molecular Biology, Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Equal contribution.
bioRxiv. 2025 Jul 31:2025.07.28.667292. doi: 10.1101/2025.07.28.667292.
Neurogenic bladder is a debilitating consequence of spinal cord injury (SCI), with few treatment options that restore voluntary voiding. Here, we show that SOX2-mediated in vivo reprogramming of NG2 glia improves bladder function in a clinically relevant mouse model of contusive SCI. NG2 glia reprogramming induces adult neurogenesis, reduces glial scarring, and significantly improves urinary performance, as measured by voiding assays and conscious cystometry. Functional recovery correlates positively with neurogenesis and negatively with glial scarring. These findings demonstrate that SOX2-mediated glial reprogramming promotes autonomic repair and offers a regenerative strategy for neurogenic bladder after SCI.
神经源性膀胱是脊髓损伤（SCI）的一种使人衰弱的后果，恢复自主排尿的治疗选择很少。在这里，我们表明，SOX2介导的体内NG2胶质细胞重编程可改善临床上相关的挫伤性SCI小鼠模型的膀胱功能。NG2胶质细胞重编程可诱导成体神经发生，减少胶质瘢痕形成，并通过排尿试验和清醒膀胱测压法测量，显著改善排尿性能。功能恢复与神经发生呈正相关，与胶质瘢痕形成呈负相关。这些发现表明，SOX2介导的胶质细胞重编程促进自主修复，并为SCI后神经源性膀胱提供了一种再生策略。
