Cold Spring Harb Protoc. 2021 Dec 1;2021(12):2021/12/pdb.prot102236. doi: 10.1101/pdb.prot102236.
Many variations of the original Coomassie Brilliant Blue staining procedure are in use. This protocol describes some selected variations on the standard procedure that give comparable and consistent staining results for proteins in the 20- to 200-kDa range.
许多原始考马斯亮蓝染色程序的变体正在使用中。本方案描述了标准程序的一些选定变体,这些变体可为 20-200 kDa 范围内的蛋白质提供可比且一致的染色结果。